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MLSC 3008 > GIT > Flashcards

GIT Flashcards

(48 cards)

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1
Q

a exotoxin that targets cells of the intestinal tract.

A

enterotoxin

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2
Q

alters the metabolic activity of the cells, result in an outpouring of electrolytes and fluid

A

enterotoxin

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3
Q

disrupts the structure of individual intestinal epithelial cells

A

Cytotoxin

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4
Q

secretory and/or absorption activities no longer function, strong host response causes further damage, pus and blood are seen in the stool

A

Cytotoxin

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5
Q

a.k.a. as “enteric fever”, diarrheal disease caused by Salmonella Typhi and S. Paratyphi

A

Typhoid

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6
Q

diarrheal disease caused by Vibrio cholera

A

chlorea

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7
Q

microbial mix of the human bowel

A

90% are anaerobes, remaining facultative anaerobes: Enterobacteriaceae, Enterococcus, yeast, GBS, GGS

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8
Q

survives stomach acid by an increased urease production, results in chronic gastritis and peptic ulcers

A

Helicobacter pylori

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9
Q

Breath test in a Dr.’s office: *C13 or *C14 labelled urea drink, patient exhales *CO2 which is measured is used for

A

Helicobacter pylori

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10
Q

(2) viruses that cause diarrhea

A

Rotavirus, Norwalk virus

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11
Q

three (3) parasites that cause diarrhea

A

Giardia lambia, Cryptosporidium sp, Entamoeba histolytica

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12
Q

Ova & Parasites transportation medium

A

SAF = sodium acetate / acetic acid / formalin
PVA = polyvinyl alcohol
fresh specimens rec.

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13
Q

collection + transport of stool specimens for C&S

A

Carey Blair Transport Media –transported at room temp
Buffered glycerol – better for Shigella
fridge/freeze, fresh is best

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14
Q

collection + transport of stool specimens for viruses

A

clean vial, see BCCDC for transport regulation

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15
Q

this plate screens for Salmonella, Shigella and contains Lactose, sucrose, & salicin, H2S

A

HEK

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16
Q

the plate screens for Yersinia enterocolitic

A

YSA and CIN

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17
Q

differential ingredients of YSA and CIN

A

Mannitol plus bile salts

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18
Q

purpose use of GN broth

A

for Shigella and salmonella

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19
Q

purpose of Selenite broth

A

Recovery of Salmonella and Shigella, inhibits coliform and fecal strep. subculture must be done before 18 hr b/c of breakthrough growth

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20
Q

plates incubated at 42 with Camp pack 48-72 hrs

Study These Flashcards
A

CAMPY, CVA with blood, Charcoal blood free PYL

21
Q

define rice water stools

Study These Flashcards
A

poo that contains flecks of whitish material (mucus and GI epithelial lining)

22
Q

BIO ID of Salmonella

MAC, HEK, MIL, Urea, Citrate, TSI

Study These Flashcards
A

MAC: NLF. HEK: clear green with black centre. MIL: +/-/+ Urea: -ive Citrate: pos. TSI K/A H2S+

23
Q

treatment and AST for uncomplicated gastroenteritis Salmonella

Study These Flashcards
A

self limiting. no abs can prolong duration of convalescent carriage

24
Q

BIO ID of Shigella

MAC, HEK, MIL, Urea, Citrate, TSI

Study These Flashcards
A

MAC: NLF HEK: clear green MIL: -/-/- Urea: neg. Citrate: neg TSA: K/A

25
treatment and AST for Shigella
amp, SxT, ciprofloxacin
26
contains deoxycholate and citrate, inhibits gp
for recovery of Shigella and Salmonella
27
BIO ID of E. coli | MAC, MIL, Urea, Citrate, TSI
MAC:LF +/+/+ urea and citrate: -ive. TSI: A/A gas
28
treatment and AST for E. coli
only for paediatric patients
29
treatment and AST for Campy
empirical. Erythromycin and fluroquinolones
30
which org appears as bull's eye clonies on CIN
Yersinia
31
BIO ID of Vibrio, Oxidase, glucose, lactose, decarbs, esculin
oxidase pos, glucose pos, lactose v, lysine and orthinine pos, arginine neg, esculin neg
32
plate incubates at 22 C/02
CIN, YSA
33
main cause f viral gastroenteritis in children
rotavirus
34
virus that is from community outbreaks, cruise ships
Norwalk virus
35
Thiosulfate Citrate Bile-salts Sucrose Agar detects
vibrio species
36
motile at 22 but not 37
Yersinia
37
oxidase pos, rice water stools
Vibrio spp
38
most common traveler's diarrhea cause
ETEC = enterotoxigenic E. coli
39
media for H. pylori
BAP with 7-10% blood, CHOC with blood, urea broth
40
causes enteric fever
Salmonella Typhi
41
causes food poisoning
Salmonella and campy
42
agents of esophagitis
Candidas albicans, HSV type 1 and CMV
43
inflammation of rectum
proctitis
44
caused extra intestinal infections, like liver disease, wound infections when occurs in water
Aeromonas
45
oxidase pos
Vibrio and aermonas
46
how to diff Vibrio and Aermonas based on TCBS agar, growth in salt births and O/129 agent
aeromonas will show NG in both and is O.129 resistant
47
how to diff vibrios from plesimonas growth in salt broth
for plesiomonas not growth in O and1% growth in 6%
48
how to diff plesimonas from aermonas by CIN
plesimonas is mannitol neg

MLSC 3008 (2 decks)

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