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Flashcards in GIT Deck (48):
1

a exotoxin that targets cells of the intestinal tract.

enterotoxin

2

alters the metabolic activity of the cells, result in an outpouring of electrolytes and fluid

enterotoxin

3

disrupts the structure of individual intestinal epithelial cells

Cytotoxin

4

secretory and/or absorption activities no longer function, strong host response causes further damage, pus and blood are seen in the stool

Cytotoxin

5

a.k.a. as “enteric fever”, diarrheal disease caused by Salmonella Typhi and S. Paratyphi

Typhoid

6

diarrheal disease caused by Vibrio cholera

chlorea

7

microbial mix of the human bowel

90% are anaerobes, remaining facultative anaerobes: Enterobacteriaceae, Enterococcus, yeast, GBS, GGS

8

survives stomach acid by an increased urease production, results in chronic gastritis and peptic ulcers

Helicobacter pylori

9

Breath test in a Dr.’s office: *C13 or *C14 labelled urea drink, patient exhales *CO2 which is measured is used for

Helicobacter pylori

10

(2) viruses that cause diarrhea

Rotavirus, Norwalk virus

11

three (3) parasites that cause diarrhea

Giardia lambia, Cryptosporidium sp, Entamoeba histolytica

12

Ova & Parasites transportation medium

SAF = sodium acetate / acetic acid / formalin
PVA = polyvinyl alcohol
fresh specimens rec.

13

collection + transport of stool specimens for C&S

Carey Blair Transport Media –transported at room temp
Buffered glycerol – better for Shigella
fridge/freeze, fresh is best

14

collection + transport of stool specimens for viruses

clean vial, see BCCDC for transport regulation

15

this plate screens for Salmonella, Shigella and contains Lactose, sucrose, & salicin, H2S

HEK

16

the plate screens for Yersinia enterocolitic

YSA and CIN

17

differential ingredients of YSA and CIN

Mannitol plus bile salts

18

purpose use of GN broth

for Shigella and salmonella

19

purpose of Selenite broth

Recovery of Salmonella and Shigella, inhibits coliform and fecal strep. subculture must be done before 18 hr b/c of breakthrough growth

20

plates incubated at 42 with Camp pack 48-72 hrs

CAMPY, CVA with blood, Charcoal blood free PYL

21

define rice water stools

poo that contains flecks of whitish material (mucus and GI epithelial lining)

22

BIO ID of Salmonella
MAC, HEK, MIL, Urea, Citrate, TSI

MAC: NLF. HEK: clear green with black centre. MIL: +/-/+ Urea: -ive Citrate: pos. TSI K/A H2S+

23

treatment and AST for uncomplicated gastroenteritis Salmonella

self limiting. no abs can prolong duration of convalescent carriage

24

BIO ID of Shigella
MAC, HEK, MIL, Urea, Citrate, TSI

MAC: NLF HEK: clear green MIL: -/-/- Urea: neg. Citrate: neg TSA: K/A

25

treatment and AST for Shigella

amp, SxT, ciprofloxacin

26

contains deoxycholate and citrate, inhibits gp

for recovery of Shigella and Salmonella

27

BIO ID of E. coli
MAC, MIL, Urea, Citrate, TSI

MAC:LF +/+/+ urea and citrate: -ive. TSI: A/A gas

28

treatment and AST for E. coli

only for paediatric patients

29

treatment and AST for Campy

empirical. Erythromycin and fluroquinolones

30

which org appears as bull's eye clonies on CIN

Yersinia

31

BIO ID of Vibrio, Oxidase, glucose, lactose, decarbs, esculin

oxidase pos, glucose pos, lactose v, lysine and orthinine pos, arginine neg, esculin neg

32

plate incubates at 22 C/02

CIN, YSA

33

main cause f viral gastroenteritis in children

rotavirus

34

virus that is from community outbreaks, cruise ships

Norwalk virus

35

Thiosulfate Citrate Bile-salts Sucrose Agar detects

vibrio species

36

motile at 22 but not 37

Yersinia

37

oxidase pos, rice water stools

Vibrio spp

38

most common traveler's diarrhea cause

ETEC = enterotoxigenic E. coli

39

media for H. pylori

BAP with 7-10% blood, CHOC with blood, urea broth

40

causes enteric fever

Salmonella Typhi

41

causes food poisoning

Salmonella and campy

42

agents of esophagitis

Candidas albicans, HSV type 1 and CMV

43

inflammation of rectum

proctitis

44

caused extra intestinal infections, like liver disease, wound infections when occurs in water

Aeromonas

45

oxidase pos

Vibrio and aermonas

46

how to diff Vibrio and Aermonas based on TCBS agar, growth in salt births and O/129 agent

aeromonas will show NG in both and is O.129 resistant

47

how to diff vibrios from plesimonas growth in salt broth

for plesiomonas not growth in O and1% growth in 6%

48

how to diff plesimonas from aermonas by CIN

plesimonas is mannitol neg