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PMLS LEC 2 (2ND SEM) > GLASSWARES > Flashcards

GLASSWARES Flashcards

(76 cards)

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1
Q

corrodes metals, irritates skin and bleaches fabrics and clothing.

A

Hypochlorite

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2
Q

useful because its impact and corrosive resistance

A

Plastic

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3
Q

Has a disadvantage: some releases ions

A

Borosilicate glass

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4
Q

low heat and chemical resistance, inexpensive, use to make disposable Container

A

Flint glass

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5
Q

Describe borosilicate glass

A

low alkaline earth content, high thermal resistance (*600°C), ideal for use, free from chemical contaminants (i.e., heavy metals)

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6
Q

Kimax and pyrex

A

Borosilicate glass

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7
Q

It is Inexpensive and disposable

A

Polyethylene

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8
Q

Example of Polyethylene

A

Petri dish

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9
Q

It is sterilizable and expensive

A

Polypropylene

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10
Q

Ex of Polypropylene

A

Autoclavable syringe filters
Autoclavable plastics

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11
Q

Flask

A

Erlenmeyer flask
Florence flask
Volumetric flask

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12
Q

Pipet

A

Serological
Mohr
Volumetric

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13
Q

BASIC LABWARES

A

1 Beaker
2. Flask - Erlenmeyerflask, Florence flask, Volumetric flask
3. Pipet - Serological, Mohr, Volumetric
4. Graduated cylinder
5. Test tubes 6. Bottles
7. Others: Stirring rod, coplin jar, forceps, tongs, test tube holder, testtube rack, pipette bulb/pipettor/aspirator, syringes, vacutainer set

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14
Q

wide-mouth, straight sided container with a pouring spout formed from the rim.

A

Beaker

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15
Q

An upright, straight-sided container with a flared base that provides stability, octagon base.

A

Graduated cylinder

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16
Q

Markings of graduated cylinder

A

By increments

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17
Q

Uses of graduated cylinder

A

non-critical volume measurement
hold 24-hour urine specimen

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18
Q

GC: read at lower meniscus

A

Light colored

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19
Q

GC: read at upper meniscus

A

Dark colored solution

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20
Q

holds liquid samples
container for test reaction

A

Test tubes

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21
Q

vacutainer tubes top

A

Stoppered

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22
Q

screw-capped tubes top

A

Screw-on

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23
Q

Kinds of top test tubes

A

Open
Stoppered (close)
Screw-on (close)

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24
Q

Kinds of bottom test tubes

A

Round (general use)
Open (ESR)
Flat (culture)
Pointed (urinalysis

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25
Routine chemical testing requiring serum; blood bank and serological tests.
Red vacutainer tube
26
Chemically clean tube; general use
Amber vacutainer tube
27
Use for Tissue culture
Pink vacutainer tube
28
Use for hematology testing
Lavender/violet
29
ANTICOAGULANT ofyellow
Sodium Polyetholesulfonate (SPS)
30
ANTICOAGULANT of black VT
Oxalate
31
ANTICOAGULANT of lavender/violet VT
EDTA
32
ANTICOAGULANT of light blue VT
Sodium citrate
33
Coagulation testing
Light blue
34
ANTICOAGULANT pf green VT
Heparin
35
Chemistry testing
Green
36
ANTICOAGULANT of royal blue VT
Sodium heparin/EDTA
37
Use for chemistry trace elements
Royal blue
38
flat bottom, sloping sides that gradually narrow its diameter so that top opening is bottle-like, plain flat opening
Erlenmeyer flask
39
flat bottom, round sides, long cylindrical neck o marking: total capacity
Florence flask
40
very long neck, pear-shaped
Volumetric flask
41
contain exact volume at specified temperature (for accurate measurement)
Volumetric flask
42
* curve fluid surface
Meniscus
43
To measure and transfer liquid or solutions.
Pipettes
44
Types pf pipettes
Measuring pipette Transfer pipette Pasteur pipette
45
Used in old determination of hemoglobin
Sahli-hellige
46
filled, allowed to drain and remaining tip fluid is blown-out deliver exact amount; not rinsed-out
To blow-out
47
Example of TBO/ To blow-out pipette
Oswald-folin -measure viscous fluid like whole blood -special pipette Serological pipette -long glass tube with uniform diameter - markings extend to the delivery tip - has two frosted band near the mouth piece
48
-refilled or rinse-out after draining by appropriate solvent -hold exact amount of liquid that must be completely transferred by rinsing for accurate measurement.
To contain(TC) / Rinse-out pipette
49
* allow liquid to drain by gravity • pipette is held vertically and the tip is place against the side of accepting vessel
To deliver (TD)
50
Measuring pipettes are divided into
Mohr pipette Serological pipette
51
the graduations on these always end before the tip
Mohr pipette
52
the graduation marks continue to the tip
Serological pipette
53
Advantages of Using Automatic Pipette:
Easy to operate Reduce technical error
54
o most common type with disposable tip, piston-operated device o fixed oradiustable volume
Eppendorf micropipette
55
• delivers cylindrical slender glass rods, for mixing manually solutions o heat-resistant
SMI Micropipettor
56
used with volumetric and standard graduated pipettes (serological of various sizes.
Electric/battery-operated pipette
57
can deliver different volumes
Battery-operated/electric pipette
58
functional from storing to mixing reagents
Test tubes
59
one of the most important apparatus
Test tubes
60
rpm means
Revolutions per minute
61
a small centrifuge used in blood banking and serology to spin serological tubes
Serofuge
62
spins special microtubes at high speed
Microfuge
63
spins capillary tubes at high speeds so that hematocrit can be measured.
Microhematocrit centrifuge
64
used to separate chylomicrons fromserum,fractionate lipoproteins, perform drug binding assays, preparation of tissue for steroid hormone receptor assay.
Ultracentrifuge
65
high speed &sampleskeep cool while being centrifuge (i.e. ultracentrifuge), use inresearch but not required for routine use.
Refrigerated centrifuge
66
container for sterilize items.
Autoclave chamber
67
surrounds the chamber and source ofsteam
Metal jacket
68
securely lock & has a seal to prevent escape ofsteam.
Door or cover
69
separate for temperature and pressure.
Gauges
70
for weighing chemicals and media
Laboratory balance
71
Never open the door unless chamber pressure is:
Zero psi
72
asingle beam with equal arms, standard weights are added manually to the right side to counter balance the object weight.
Double pan (DIRECT COMPARISON)
73
arms are unequal in length, object is placed on the short arm pan and arestoring force mechanicallyapplied tothe other armuntil indicator is balanced.
Single Pan (SUBSTITUTION)
74
Important Parts of the Autoclave:
Autoclave chamber Metal jacket Door / cover Gauges
75
TEMPERATURE CONTROLLED CHAMBERS
1) Above ambient or room temperature a. oven b. water bath (heating bath) c. incubator 2) Cold temperature a. refrigerator b. freezer
76
Principle of digital weighing scale
Electromagnetic force

PMLS LEC 2 (2ND SEM) (4 decks)

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