Glycolysis Flashcards

(54 cards)

1
Q

What is the first step of cellular respiration?

A

Glycolysis

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2
Q

How is breathing related to cellular respiration?

A
  1. In breathing, the lungs exchange CO2 and O2 between the body and the atmosphere
  2. In cellular respiration, the cells consume the O2 in extracting the energy from food and release CO2 as a waste product
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3
Q

Cellular respiration is a term that refers to?

A

The biochemical pathway by which cells release energy from the chemical bond of the food molecules and provide that energy for the essential processes of life

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4
Q

Prokaryotic cells carry out cellular respiration __________. Meanwhile, eukaryotic cells carry out cellular respiration in the ______________.

A

Cytoplasm; Mitochondrion

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5
Q

Similarities of Aerobic and Anaerobic Respiration

A
  1. Both undergo glycolysis in the cytoplasm of the cell.
  2. Both undergo substrate-level phosphorylation and oxidative phosphorylation and chemiosmosis in producing ATP molecules.
  3. Both split the 6- carbon glucose into two molecule of pyruvate, the three carbon molecule.
  4. Both involved a series of enzyme-controlled reactions that takes place in the cytoplasm.
  5. Both use NAD+ (nicotinamide adenine dinucleotide), a redox coenzyme that accepts two electrons plus a (H+) that becomes NADH
  6. Both performed by eukaryotic and prokaryotic cells
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6
Q

Main Functions of Aerobic Respiration and Anaerobic Respiration

A

Aerobic Respiration: Production of ATP from food such as carbohydrate, lipid and protein

Anaerobic Respiration: Production of ATP without the use of oxygen

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7
Q

Site of reaction of Aerobic Respiration and Anaerobic Respiration

A

Aerobic Respiration: Cytoplasm and Mitochondrion

Anaerobic Respiration: Cytoplasm

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8
Q

Production of ATP of Aerobic Respiration and Anaerobic Respiration

A

Aerobic Respiration: 36 to 38 ATP per glucose molecule

Anaerobic Respiration: 2 ATP per glucose molecule

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9
Q

Sustainability of Aerobic Respiration and Anaerobic Respiration

A

Aerobic Respiration: Long Term

Anaerobic Respiration: Short Term

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10
Q

Production of Lactic Acid of Aerobic Respiration and Anaerobic Respiration

A

Aerobic Respiration: Does not produce

Anaerobic Respiration: Produces

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11
Q

Oxygen Requirement of Aerobic Respiration and Anaerobic Respiration

A

Aerobic Respiration: Needs O2

Anaerobic Respiration: Does not need O2

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12
Q

Recycling of NADH of Aerobic Respiration and Anaerobic Respiration

A

Aerobic Respiration: Through the electron transport system

Anaerobic Respiration: In lactic acid fermentation, (i.e, muscle cells; in alcohol fermentation (pyruvate is converted to carbon dioxide and ethanol)

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13
Q

Participating Cells of Aerobic Respiration and Anaerobic Respiration

A

Aerobic Respiration: Most cells

Anaerobic Respiration: Yeast, other fungi, prokaryotes, and muscle cells

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14
Q

Glycolysis occurs where?

A

It occurs in the cytoplasmic fluid of the cell, that is, outside the organelles

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15
Q

Where does Krebs Cycle occur?

A

Within the mitochondria

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16
Q

What does the Krebs Cycle do?

A

It completes the breakdown of glucose by decomposing a derivative of pyruvic acid to carbon dioxide

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17
Q

Three main stages of Respiration

A
  1. Glycolysis
  2. Krebs Cycle
  3. Electron Transport Chain
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18
Q

What happens in the Electron Transport Chain?

A

It obtains electrons from the hydrogen carrier NADH, the reduced form of NAD+

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19
Q

FAD also shuttles some ________ from the Krebs cycle to the electron transport chain. The reduced form of FAD is FADH2

A

Electrons

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20
Q

Glycolysis and the Krebs cycle
are energy-releasing stages that?

A

Extract electrons from food molecules
while breaking these molecules down
to CO2

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21
Q

NAD+ and FAD temporarily capture the electrons and relay them to where?

A

The top of the Electron Transport Chain

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22
Q

What is the reduced form of FAD?

23
Q

The chain uses the _______________ of
electrons from NADH and FADH2 to O2
to pump H+ ions across a membrane.

A

Downhill Flow

24
Q

Glycolysis means?

A

“Splitting of Sugar”

25
How does Glycolysis begin?
Glycolysis begins with a single molecule of glucose and concludes with two molecules of another organic compound, pyruvic acid
26
What is Glycolysis?
It is the metabolic process that serves as the foundation for both aerobic and anaerobic cellular respiration
27
What happens to glucose in Glycolysis?
It is converted into pyruvate
28
Glucose is a six-membered ring molecule that is found where?
In the blood Additional Info: Glucose is usually a result of breakdown of carbohydrates into sugars
29
Overall Equation of Glycolysis
Glucose + 2 NAD+ 2 ADP + 2Pi -> 2 Pyruvate + 2 NADPH + 2 ATP + 2H+ + 2 H20
30
In Glycolysis, there is a net gain of _____ ATP and, ____ pyruvates are formed
two; two
31
The first step in glycolysis is the conversion of D-glucose into glucose-6-phosphate. This is catalyzed by what enzyme?
Hexokinase
32
When the glucose ring is phosphorylated, how many molecule/s of ATP is consumed?
1
33
The second reaction of glycolysis is the rearrangement of glucose-6-phosphate into fructose-6-phosphate by what enzyme?
Glucose phosphate isomerase (Phosphoglucose isomerase)
34
What does G6P mean?
Glucose-6-phosphate
35
What does F6P mean?
Fructose-6-phosphate
36
What does FBP mean?
Fructose 1,6-bisphosphate
37
______________, with magnesium as a cofactor, changes fructose-6-phosphate into fructose 1,6-bisphosphate.
Phosphofructokinase
38
Fructose-6-phosphate is converted to?
Fructose 1,6-bisphosphate (FBP)
39
What is the enzyme that catalyzes the reaction between F6P and FBP?
Phosphofructokinase (PFK)
40
The enzyme ________ splits fructose 1, 6-bisphosphate into two sugars that are isomers of each other. These two sugars are dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3- phosphate (GAP) or Phosphoglyceraldehyde (PGAL)
Aldolase
41
What does DHAP mean?
Dihydroxyacetone Phosphate
42
What does PGAL mean?
Phosphoglyceraldehyde
43
What does GAP stand for?
Glyceraldehyde-3-phosphate
44
The enzyme ______________________ rapidly inter-converts the molecule dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3phosphate or phosphoglyceraldehyde. Glyceraldehyde-3-phosphate or phosphoglyceradehyde is removed and used in next step of Glycolysis
Triosphosphate isomerase or Phosphotriose isomerase
45
_________________ dehydrogenates and adds an inorganic phosphate to glyceraldehyde 3-phosphate or phosphoglyceraldehyde producing 1, 3- bisphosphoglycerate
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
46
What does GAPDH mean?
Glyceraldehyde-3-phosphate dehydrogenase
47
__________________ transfers a phosphate group from 1,3-bisphosphoglycerate to ADP to form ATP and 3 phosphoglycerate
Phosphoglycerate kinase or Phosphoglyceric acid kinase
48
The enzyme ____________________ mutase relocates the P from the 3- phosphoglycerate from the 3rd carbon to the 2nd carbon to form 2- phosphoglycerate
Phosphoglycerate Mutase or Phosphoglycerate Acid Mutase
49
The enzyme _______ removes a molecule of water from 2 phosphoglycerate to phosphoenolpyruvate (PEP) or phosphoenol pyruvic acid
Enolase
50
What does PEP mean?
Phosphoenolpyruvate
51
How does Enolase work?
It works by removing a water group, or dehydrating the 2 phosphoglycerate
52
The enzyme _____________________________ transfers a P from phosphoenolpyruvate (PEP) or phosphoenolpyruvic acid to ADP to form pyruvic acid and ATP
Pyruvate Kinase or Pyruvic Acid Kinase
53
How many ATPs are produced in the steps of Glycolysis?
Step 1 and 3 = -2ATP Step 7 and 10 = +4 ATP Net “visible” ATP produced = 2 ATP
54
A single glucose molecule in glycolysis produces a total of ___ molecules of pyruvate, ___ molecules of ATP, ___ molecules of NADH and ___ molecules of water.
two; two; two; two