Glycolysis Steps

Question 1

Step 1

Answer 1

Glucose is phosphorylated to glucose-6-phosphate (G-6-P), using energy and phosphate from ATP

• Same reaction that keeps glucose inside cells
• G-6-P rearranges to form fructose-6-phosphate (F-6-P)

Question 2

Step 2

Answer 2

F-6-P is phosphorylated to form fructose -1,6-diphosphate, using energy and phosphate from ATP

Question 3

Step 3

Answer 3

F-1,6-diP splits into two 3-carbon molecules, dihydroacetone phosphate (DHAP) and 3-phosphoglyceraldehyde (3-PGA).

• Each DHAP is converted to 3-PGA.
• From this point, two 3-carbon molecules must be catabolized to account for 1 glucose molecule

Question 4

Step 4

Answer 4

Inorganic phosphate from the cytosol is added to each 3-PGA molecule. Two hydrogen atoms are transferred from it to NAD+ to form NADH + H+ and 1,3-diphosphoglycerate (1,3-DPG)

Question 5

Step 5

Answer 5

Phosphate and energy from 1,3-DPG are added to ADP to make ATP and 3-phosphoglycerate, which becomes 2- phophoenolpyruvate

Question 6

Step 6

Answer 6

Water is removed from 2-phosphoglycerate to form phosphoenolpyruvate (PEP)

Question 7

Step 7

Answer 7

PEP is converted to pyruvate and phosphate and energy is added to ADP to form ATP. The fate of pyruvate depends on whether oxygen is available