God Send

Question 1

What regulatory mechanism is exemplified by a decrease in activity of an enzyme early in a metabolic pathway by the final product of the pathway

Answer 1

Feedback inhibition

Question 2

Phosphorylation of a eukaryotic initiation factor eIF-2 regulates gene expression by affecting which general process

Answer 2

Protein synthesis

Question 3

Aminoacyl-tRNA synthetases

Answer 3

Recognize specific amino acids and specific tRNA’s

Question 4

Which compound is not directly derived from pyruvate

Answer 4

Phosphoenolpyruvate

Question 5

What two substrates are responsible for the generation of ATP in the payoff phase of glycolysis

Answer 5

Phosphoenolpyruvate and 1,3-bisphosphogycerate

Question 6

Which of the following statements BEST describes allolactose’s role as an inducer of the lac operon

Answer 6

A ligand that inactivates the DNA binding capacity of the lac repress or protein

Question 7

In the liver, which glycolysis enzyme is not a critical point of metabolic regulation in glucose catabolism

Answer 7

Phosphoglycerate dephosphorylation of 1,3-bisphosphoglycerate

Question 8

Glycolysis is an example of which type of metabolic pathway

Answer 8

Linear

Question 9

Which of the following statements about type II restriction endonuclease enzymes is false

Answer 9

Many make staggered (off centered) cuts of both DNA strands within their recognition sequences

Question 10

Which class of nuclei acid serves as an “adapter” for the activation and selection of amine acids used in protein synthesis

Answer 10

tRNA

Question 11

Which component is NOT required in a polymerase chain reaction (PCR)

Answer 11

DNA ligase

Question 12

Which bacterial rRNA, when combined with ribosomal proteins to make the 50S subunit, possesses peptidyl transferase enzyme activity

Answer 12

23s

Question 13

A dietary deficiency of which vitamin involved in oxidation-reductions causes dermatitis among other symptoms

Answer 13

Nicotinic acid

Question 14

Attenuation, or coupled transcription/translation regulation, involves formation of alternative RNA transcript stem-loop structures by directing amino acid abundance. Which of the following amino acid abundance in the attenuation mechanism

Answer 14

70s ribosome

Question 15

In most cases, more than one codon specifies for a single amino acid. This describes the genetic code as being

Answer 15

Degenerate

Question 16

Which of the following eukaryotic RNAP enzymes is unaffected by the mushroom toxin alpha-amanitin

Answer 16

RNAP I (most affected is II)

Question 17

What metabolite is oppositely regulated by glucagon and insulin to effect glycolysis or gluconeogenesis

Answer 17

Fructose 2,6-bisphosphate

Question 18

Which eukaryotic mRNA post transcriptional process is coordinated by a physical association with the carboxy terminal domain (CTD) of eukaryotic RNAP II

Answer 18

All of the above.
5’ cap addition to eukaryotic mRNA, 3’ dnd poly A addition to eukaryotic mRNA, intron removal and exon-exon splicing of eukaryotic mRNA.

Question 19

Which one of the following antibiotics inhibits bacterial translation initiation by interfering with Fmet-tRNA^met, binding to P site of the 30 s ribosome

Answer 19

Streptomycin

Question 20

Which nucleotide substrate is post transcriptionally added to the 5’ nucleotide of eukaryotic mRNAs by forming an unusual 5’,5’ triphosphate bond

Answer 20

GTP

Question 21

Which elongation factor mimics a changed tRNA and is capable of trans locating a peptidyl-tRNA from the 70s ribosome A site to the P site

Answer 21

EF-G-GTP

Question 22

Which of the following enzymes is/are used to compete the 5’ cap structure on eukaryotic mRNAs

Answer 22

All of the above
2’-O-methyltransferase, guanine-7-methyltransferase, guanlylytransferase

Question 23

What enzyme isoform in the liver has lower affinity for glucose and helps maintain blood glucose levels

Answer 23

Hexokinase IV

Question 24

CAMP binds to cAMP receptor protein (CRP), cAMP-CRP binds to DNA near the lac operon promoter. What type of transcriptional regulator is cAMP-CRP

Answer 24

Activator

Question 25

You are given an uncharacterized mRNA nucleotide sequence and asked to find the longest open reading frame using the standard genetic code. The number of potential re…

Answer 25

3

Question 26

Which of the following best describes the purpose of the first, or 5’ nucleotide (“wobble”) of a tRNA anticodon

Answer 26

Expands the number of mRNA codons a tRNA will recognize

Question 27

After the first “charged” or “activated” tRNA is placed in the appropriate robosomal site for translation, EF-Tu-GTP complex carries all subsequent “charged” tRNAs to which site of the ribosome

Answer 27

A site

Question 28

Which glucose transporter is responsible for insulin-dependent uptake of glucose in fat and muscle

Answer 28

GLUT4

Question 29

What compound is both a product and an allosteric activator of phosphofructokinase-1

Answer 29

AMP

Question 30

The genetic code, or rules by which DNA sequence information is translated into Amino acid sequence, consists of three NT sequence blocks (codons) that…

Answer 30

Non-overlapping

Question 31

Which of the following statements about the polymerase chain reaction is FALSE

Answer 31

DNA amplification is strictly linear in magnitude with respect to the number of cycles of DNA synthesis

Question 32

The mRNA sequence 5’-GCU ACG UCC-3’ is being translated by a ribosome. Which of the following sets of anticodons represent the three sequential aminoacyl-tRNAs

Answer 32

5’-GGA-3’, 5’-CGU-3’, 5’-GCG-3’

Question 33

Which substrate or metabolite has the highest standard free energy of phosphate hydrolysis

Answer 33

Phosphoenolpyruvate

Question 34

Deficiency in the ability to convert which monosaccharide from lactose prevents its entry into glycolysis and causes its reduction to a product that causes disease

Answer 34

Glucose

Question 35

Which statement is true concerning the Sanger method of DNA sequencing

Answer 35

Fluorescent ddNTPs incorporate into the template DNA for detection by sequencing instruments