Gram Negative and Other Bacteria

Question 1

Neisseria gonorrhoeae

Answer 1

gram-negative diplococci, ferment glucose, no capsule

produce IgA protease

Question 2

Neisseria meningitidis

Answer 2

gram negative diplococci, ferment glucose and maltose, capsule

Question 3

Haemophilus influenzae

Answer 3

small gram-negative coccoid rods

aerosol transmission; produces IgA protease

Question 4

Legionella pneumophila

Answer 4

gram-negative rod - use silver stain

grow on charcoal yeast extract with iron and cysteine

Question 5

Pseudomonas aeruginosa

Answer 5

gram-negative aerobic rods

non-lactose fermenting, oxidase positive, blue-green pigment, grape-like odor

Question 6

Klebsiella

Answer 6

gram-negative rod, fast lactose fermenter

intestinal flora

Alcoholics & diabetics

red “currant jelly” sputum

Question 7

Salmonella

Answer 7

gram negative rod, lactose nonfermenter, oxidase negative

Question 8

Shigella

Answer 8

gram negative rod, lactose nonfermenter, oxidase negative

Question 9

Campylobacter jejuni

Answer 9

gram-negative comma shaped

grows in 42 C, oxidase positive

Question 10

Vibrio cholerae

Answer 10

gram-negative comma shape

Question 11

Yersinia enterocolitica

Answer 11

gram-negative

Question 12

Helicobacter pylori

Answer 12

gram-negative curved rod, urease positive, oxidase positive

Question 13

Treponema pallidum

Answer 13

sphirochete - dark-field microscopy

Question 14

Rickettsia rickettsii

Answer 14

obligate intracellular organisms that need CoA and NAD+

Question 15

Chlamydia trachomatis

Answer 15

obligate intracellular organism that cause mucosal infections

Question 16

Mycoplasma pneumoniae

Answer 16

no cell wall –> no gram stain (membrane contains sterols)

grown on Eaton’s agar