Gram positive and negative

Question 1

Difference between Gram positive and negative?

Answer 1

Question 2

Steps of Gram staining?

Answer 2

• Applying a primary stain (crystal violet) to a heat-fixed smear of a bacterial culture.
• The addition of iodine, which binds to crystal violet and traps it in the cell,
• Rapid decolorization with alcohol or acetone, and
• Counterstaining with safranin.

Question 3

Steps of gram staining in pictures

Answer 3