Gram-Positive Bacilli

Question 1

displays Babes-Ernst granules

Answer 1

Corynebacterium sp.

Question 2

grows on Loeffler’s serum agar

Answer 2

Corynebacterium sp.

Question 3

CTBA agar

Answer 3

cystine-tellurite blood agar

Question 4

Corynebacterium sp. shows this on CTBA agar

Answer 4

brown halo

Question 5

used to measure toxigenicity of C. diphtheriae

Answer 5

Elek’s test

Question 6

diphtheria toxin: fragment A function

Answer 6

cytotoxic, inhibits protein synthesis

Question 7

the diphtheria antitoxin is made in ______

Answer 7

horses

Question 8

diphtheria toxin: fragment B function

Answer 8

binds to cell membrane receptors to mediate entry of fragment A

Question 9

diphtheria toxin production is inversely proportional to ______________

Answer 9

iron availability

Question 10

Corynebacterium morphology under microscope

Answer 10

club-shaped

Question 11

the two forms of diphtheria

Answer 11

respiratory and cutaneous

Question 12

how diphtheria toxin causes death

Answer 12

toxin enters blood, systemic infection, cardiac failure

Question 13

Listeria morphology under microscope

Answer 13

coccobacillus

Question 14

humans are exposed to listeria in ________ (2 things)

Answer 14

dairy products and processed meat

Question 15

listeriolysin O function

Answer 15

hemolytic and cytotoxic; allows organism to survive inside macrophages

Question 16

Listeria surface protein p60 function

Answer 16

induces phagocytosis through adhesion and penetration into mammalian cells

Question 17

effects of listeriosis in pregnant women

Answer 17

premature labor and spontaneous abortion

Question 18

Listeria grows at this unusual temperature

Answer 18

4 C

Question 19

Listeria shows this kind of motility at 25 C

Answer 19

umbrella

Question 20

Listeria shows this kind of motility in a wet prep

Answer 20

tumbling

Question 21

Listeria is catalase-_______

Answer 21

positive

Question 22

Corynebacterium is catalase-_______

Answer 22

positive

Question 23

Listeria CAMP test produces ______-hemolysis

Answer 23

block

Question 24

Arcanobacterium shows this on agar

Answer 24

black dot under colonies

Question 25

Arcanobacterium and Erysipelothrix are catalase-_______

Answer 25

negative

Question 26

Gardernella vaginalis stains gram-_______

Answer 26

variable

Question 27

grow G. vaginalis on these types of agar (2 types)

Answer 27

human blood bilayer Tween agar (HBT) and V agar

Question 28

atmospheric requirement for Nocardia

Answer 28

aerobic

Question 29

Nocardia staining properties

Answer 29

weakly acid-fast, gram-variable

Question 30

this genus looks like a molar tooth on agar

Answer 30

Actinomadura

Question 31

Actinomadura colonies are white but can _________

Answer 31

become red

Question 32

develops salmon-pink pigment after 4-7 days

Answer 32

Rhodococcus

Question 33

N. cyriaciageorgica and N. farcinica cause

Answer 33

necrosis with abscesses

Question 34

N. braziliensis causes

Answer 34

actinomycotic mycetomas

Question 35

Streptomyces smell

Answer 35

woodsy

Question 36

Streptomyces appearance

Answer 36

waxy, glabrous, heaped

Question 37

B. anthracis capsule is composed of ________

Answer 37

antiphagocytic D-glutamate

Question 38

cutaneous anthrax appearance

Answer 38

painless black pustule

Question 39

pulmonary anthrax symptoms

Answer 39

fever, fatigue, malaise; then respiratory distress, coma, death w/n 24 hrs

Question 40

four types of anthrax

Answer 40

cutaneous, pulmonary, gastrointestinal, injectional

Question 41

B. anthracis morphology (agar and microscope)

Answer 41

agar: medusa-head/beaten egg white colonies, string of pearls when exposed to penicillin microscope: bamboo

Question 42

B. anthracis - hemolysis and motility

Answer 42

nonhemolytic, nonmotile

Question 43

B. cereus - hemolysis and motility

Answer 43

beta-hemolytic, motile (flagella)

Question 44

Bacillus is catalase-______

Answer 44

positive

Question 45

Bacillus causes these diseases

Answer 45

food poisoning and eye infections

Question 46

2 types of Bacillus food poisoning

Answer 46

emetic (fried rice) and diarrheal (meat, poultry, soup)

Question 47

spore-forming gram-positive rods

Answer 47

Bacillus sp.

Question 48

Erysipelothrix causes ________

Answer 48

painful swelling of hands