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Flashcards in Gram Positive DSA Deck (17):
1

Staphylococci morphology metabolic activity and virulence factors?

gram (+) cocci in clusters.

catalase positive

virulence: polysac capsule (resist phagocytosis)
Biofilm slime layer adheres to artificial materials

2

S. Aureus, special characteristics and diseases?

Coagulase (+), B HEMOLYTIC

Diseases: Scalded skin syndrome & bullous impetigo (exfoliative A & B toxins)
Acute Endocarditis
Toxic shock syndrom
Food poisoning.

3

S. epidermidis, special characteristics, and diseases

NON HEMOLYTIC, coagulase (-)

subacute endocarditis
infection of catheters and prothesis via biofilm.

4

Staphylococcus saprophyticus characteristics and diseases.

Coagulase -, nonhemolytic

UTI in young females

5

Dx of staphylococcus

Colony hemolysis on blood agar: B hemolytic = S. aureus

Catalase test = (+) for staphylococci

Coagulase = + = S. Aureus

6

Treatment of staphylococci

Antibiotics

MRSA- methycillin resistant staph. aureus vi B lactamases
VRSA- Vanco '' '' via plasmid

7

streptococci morphology

Gram (+) cocci in chain (except S. pneumonia- lancet shaped

Catalase negative

8

Streptococci pyogenes characteristic and diseases?

Group A

B hemolytic, M proteins and hyaluronic acid capsule (resist phagocytosis)

Disease: #1 cause bacterial pharyngitis and tonsillitis
Scarlet fever (via erythrogenic exotoxins)
Necrotizing fasciitis

post auto immune diseases: Rheumatoid A., Rheumatic valvular disease, post strep glomerular nephritis

9

Streptococci agalactiae

Group B

B hemolytic, capsule

Leading cause of neonatal septicemia, meningitis & pneumonia

10

Streptococci bovis or s. equinus

Group D

non or a hemolytic

11

Strepto pneumonia

a hemolytic, capsule

#1 cause of pneumonia in adults

#1 cause of oditis media in children

12

Strepto viridans

a hemolytic, lives in gums

13

Dx of streptococci

Blood agar hemolysis:
a hemolytic: pneumonia, viridans, and some of D
B hemolytic: pyogenes, agalactiae

Capsular Quellung reaction for pneumonia
Optichin sensitivity for pneumonia

14

Prevention and treatment for streptococci

Pneumococcal vaccine: polyvalent anticapsule polysaccharides

Antibiotics

15

Enterococci

gram positive diplococci in short chains

Catalase negative, non-hemolytic

16

Enterococci virulent factors

Capsule

biofilms

Intrinsic B lactam and amino glycoside resistance
Vanco resistance

17

E. Faecalis

Dx:

Rx:

Part of normal flora, non hemolytic

Dx: Cultures on blood and chocolate agar ( aerobic an anaerobic)

Rx: Abx, resistant to penecilin, cephalosportins & carbapenems, possible resistant to vanco, aminoglycosides and other.