Gram + Sphere Project Flashcards

1
Q

For the growth at 45 experiment, what did it test for?

A

growth at elevated temperature

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2
Q

what gives a pos/neg result in the 45 degree experiment?

A

pos: turbid/ cloudy (growth)
neg: clear broth (no growth

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3
Q

what tool did we use to inoculate the 45 degree experiment?

A

cotton swab

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4
Q

what does the urea agar slant test for?

A

urease production

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5
Q

what tool was used to inoculate the urea agar slant?

A

loop

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6
Q

what is the original media color of urea agar slant?

A

peach color

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7
Q

what gives a pos/neg result in the urea agar slant?

A

pos: hot pink color change = ^ pH
neg: yellow color change = v pH

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8
Q

what was the reaction for the substrate and products of the urea agar slant?

A
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9
Q

what does the Vogel Johnson Agar test for?

A

mannitol fermentation and coagulase production

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10
Q

what chemicals are in Vogel Johnson Agar?

A

tellurite and lithium chloride

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11
Q

what tool and what technique is used within the Vogel Johnson Agar experiment?

A

cotton swab
zag plating technique

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12
Q

what shows a pos/neg result for mannitol fermentation within the Vogel Johnson Agar?

A

pos: yellow color change
neg: remains red

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13
Q

what shows a pos/neg result for coagulase production within the Vogel Johnson Agar?

A

pos: black colonies (tellurite gets reduced to tellurium)
neg: no black colonies

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