Haemotology Flashcards
(25 cards)
What is the EDTA tude?
The purple tube that chelates calcium, stopping clotting for blood analysis.
Common blood sample artifacts.
Clots, Platelets, lipaemia, Heinz bodies, nRBC
What should you ensure not to do when storing a blood sample.
Do not refridgerate as it causes lysis. Do not freeze as it condenses the cells and makes water artifacts. A delay in sampling handling can cause swollen cells and cell lysis.
The key points of Erhthrocytes.
It is produced mainly in bone marrow, taking 5 days to make reticulocytes. Extremedullary haematopoeisis may occur in cases of increased demand.
Half lifes:
Cat - 70 days
Dog - 110 days
What do you look at during a RBC evaluation.
Haemocrit, PCV, RBC count, MCV, Peripheral blood smear exam(morphology).
Neutrophil life cycle.
Mature- band - metsmyeloyte - myelocyte
What suffix would you use for a decrease in cells.
-paenia
Key points of platelets.
Thromocytes are some of the largest cells in the body, also being involved in homeostasis. All platelets are thromocytes but not all thrombocytes are platelets.
What is thromobocytopaenia and its clinical signs.
Thromobocytopaenia is spontaneous bleeding that happens at extremely low counts. Its clinical signs include: Petechiae(pinpoint bruising), Ecchymosis (bruising), and melaena.
It is diagnosed by platelet counts and a blood smear examination.
What are the clinical signs for aneamia?
Pale colour of the mucous membrane, lethargy, exercise intolerance, tachycardia, tachypnoea, heart murmur, collapse.
difference in -philia and -cytosis.
-philia is for granulocytes only. Cytosis is for all over cells. They both mean an increase.
What is MCV?
MCV is the mean cell volume in the blood. Normocytic - normal, microcytic - low, macrocytic - high.
What is MCH?
MCV is the mean cell haemoglobin. Hypochromic, normochromic, and hyperchromic.
What is anisocytosis?
Different cell sizes.
What is polychromia?
Different cell colours.
Which tube is the serum/plain tube?
The red and pink tube. This is normally used in biochemistry for hormonal essays, serology, and clotting. The clotting in this tube takes time compared to others.
Which is the heparin tube?
The green tube. This is used for in-house chemistry, and is an anti-coagulant.
Which is the citrate tube?
The light vlue tube. This had coagulation profiles and is an anti-caogulant.
Which is the fluoride oxidate tube?
The yellow and gret tube. This is used for glucose examinations and is an anti-coagulant.
What are the liver paramters?
ALT, AST, ALP
What are the muscle parameters?
CK , AST
What are the kidney parameters?
SDMA, filtrates such are urea and creatine. You should do a urinalysis for the kidneys.
What are the pancreas parameters?
PLI, TLI