Hand Washing Lab Flashcards
(30 cards)
Normal Flora
- Staphylococcus epidermidis
- Corynebacteria
- Staphylococcus aureus (also transient)
- Propionibacterium
- Streptococcus pyogenes
- Candida albicans (yeast)
Group A Streptococci
strep throat
Staphylococcus aureus
acne
Klebsiella
wound infections
Clostridium difficile
colitis
Haemophilus
pink eye
Pseudomonas aeruginosa
wound infection (blue-green pus)
E. coli
diarrhea and/or uti
Bacteroides
ear infection or vaginitis (after BM and not washing hands)
Shigella
diarrhea
Haemophilus influenzae
pneumonia
Bacillus species
regular contaminant from soil
What is the purpose of washing hands?
To prevent the spread of disease
What is the purpose of the normal flora on our skin?
To protect us from the invasion of pathogens
3 groups of normal flora
- Diptheroids
- Staphylococci
- Yeast and Fungi
Diptheroids
- gram positive
- non-pathogenic
- Propionibacterium acnes > in pores and hair follicles - breaks down oily substances
Staphylococci
- inhibit pathogens from establishing any presence on skin
- Staph epidermidis
Yeast and Fungi
degrade lipid secretions
Transient Bacteria
- bacteria we pick up from environment
- temporary contaminants
- easily removed
Hand Sanitizer
- 65% ethanol
- dries bacteria from the inside out
- coagulates proteins
Alcohol-Free Sanitizer (Wet Ones)
- 0.3% benzalkonium chloride
- targets cell membrane
Povidine iodine scrubs
- 13% iodine
- iodine crystals “pop” bacteria
- bacteria ruptures and dies
Antibacterial soap
0.3% chloroxylenol
Kirby Bauer Disk of Diffusion Method
-tests sensitivity of antibiotics and disinfectants
