HCLD 101

Question 1

what % atmospheric oxygen should trigger lab alarm

Answer 1

18%

Question 2

What is the oxygen level at which a worker can asphyxiate and die

Answer 2

10%

Question 3

UN3373

Answer 3

category B label for non hazardous biological specimens

Question 4

UN2814

Answer 4

infectious substances only to humans

Question 5

UN2900

Answer 5

infectious only to animals

Question 6

how should ethanol be labelled

Answer 6

flammable

Question 7

what is a Cusum plot

Answer 7

cumulative sum chart to detect deviation from the mean

Question 8

what are Westgard rules

Answer 8

statistical rules to determine under what circumstances a run should be rejected

Question 9

what is a levy-jennings plot

Answer 9

plotting results against the mean. 2sd either side

Question 10

medicare is for ?

Answer 10

old people

Question 11

medicaid is for?

Answer 11

poor people

Question 12

CMS

Answer 12

center for medicare and medicaid services

Question 13

what is stark law

Answer 13

must not refer to lab that owns another lab/who has interests

Question 14

age discrimination in employment act

Answer 14

ADEA 1967

Question 15

the perported benefits of co-culture are all of the following except: a: prod of ammonia that stims embryo devel bprod of embryotoxic factors c: rel growth factors into CM c; removal of toxins from medium

Answer 15

A

Question 16

which of the following may be added to proteins in human serum: q vit/gluc and fatty acids b: gluc, hormones and vit c; fatty acids, glu and hormaones d: hormones, vit and fatty acids

Answer 16

D

Question 17

glutathione is a marker of what in the oocyte

Answer 17

cytoplasmic maturation

Question 18

sperm gain potential to fertilize at which stage

Answer 18

during passage through female tract

Question 19

what are the sequences of events in the egg after fert:

Answer 19

intracellular Ca release-meiotic reactivation-separation of chromatids

Question 20

preimplantation cleaveg is charcterised by what frequenct

Answer 20

initially slow follwed by more rapid cleavage

Question 21

implantation is categorized by what events

Answer 21

hatching-apposition-invasion-syncytialization

Question 22

2 main functions of amniotic fluid are:

Answer 22

shock absorber and buffer temp changes

Question 23

primary follicle devl involves which changes:

Answer 23

granulosa cell prolif and increase in oocyte vol

Question 24

LH surge is triggered under what hormonal conditions

Answer 24

high E2 decreasing FSH

Question 25

how many eggs left at puberty

Answer 25

400,000

Question 26

how many eggs at birth

Answer 26

2,000,000

Question 27

how many eggs at menopause

Answer 27

0

Question 28

primordial follicle is unstimulated

Answer 28

primary follicle has started stim

Question 29

In an adult human female, the development of a primordial follicle containing an oocyte to a preovulatory follicle takes how long?

Answer 29

in excess of 120 days.

Question 30

primary oocyte lives in the primordial follicle, is it haploid or diploid

Answer 30

diploid

Question 31

dictyate or dictyotene

Answer 31

prolonged resting of primary oocyte phase up to 50 years

Question 32

what do connexin proteins do in oocyte?

Answer 32

gap juction proteins maintain cooyte arrest

Question 33

what is the muscosal layer of the vag mainly comprised of

Answer 33

stratified squamous eplithelium and no secretory cells

Question 34

endometrium is in 3 layers. 2 are sensitive to steroid stim and are called stratum functionalis. what are the 2 layers called:

Answer 34

stratum spongiosum and stratum compactum

Question 35

how many eggs by 5th month of gestation

Answer 35

6-7 million

Question 36

ratio of ICM to TE cells:

Answer 36

1:3

Question 37

primordial follicles-primary follicles-secondary follicles what is egg stage in each

Answer 37

primary oocyte-secondary oocyte only at ovulation

Question 38

is a morula charachterised by a polarized outer cell population?

Answer 38

yes

Question 39

when does developmental failure occur most often?

Answer 39

3rd meiotic division and blastulation

Question 40

best indictaor of embryo competency

Answer 40

ICM

Question 41

aneuploidy

Answer 41

multinucleation

Question 42

what is a centimorgan

Answer 42

distance between chromosome positions for which there is 1% chance of a crossover

Question 43

are mitochondrial disorders typically mosaic for the condition

Answer 43

YES

Question 44

does the presence of h2O2 prod lactobacillus enhance implantation?

Answer 44

YES

Question 45

Does vit cryoprotectant have high or low MW

Answer 45

HIGH

Question 46

what is cell lysis caused by during thawing

Answer 46

osmotic stress

Question 47

FSH

Answer 47

secreted by ant pit in response to GnRH from hypothalamus.

Question 48

E2

Answer 48

secreted by granulosa cells which use androgens from theca cells to make E2

Question 49

action of E2

Answer 49

grows follicles and stimulates endo thickening

Question 50

P4

Answer 50

prod by CL formed after ovulation and then by placenta

Question 51

hCG

Answer 51

prod by trophectoderm

Question 52

LH

Answer 52

prod by ant pit. Surge initiates ovulation and occurs in response to high E2 levels

Question 53

FSH receptors on what cells

Answer 53

granulosa and sertoli cells

Question 54

sertoli cells do what?

Answer 54

make inhibin (negative feedback) and androgen binding protein

Question 55

leydig cells produce what?

Answer 55

testosterone in response to LH

Question 56

hormonal control of spermatogenesis

Answer 56

Question 57

semen cut off variables for all WHO editions

Answer 57

Question 58

In the Dr. Menkveld presentation, no WHO 5th vs. WHO 4th Morphology criteria differences are mentioned, only a lower reference limit. Strict criteria is used for morphology assessment and grading in both manuals. * The concept of normal spermatozoon by WHO 4th and 5th manuals are the same. * Most of WHO 4th and 5th Morphology criteria are the same. * There are the slight but not significant differences in some dimensional details that were updated in the WHO 5th manual. • The WHO 4th manual Morphology reference value was not final and was updated in the WHO 5th edition. The overall conclusion is that the WHO 4th and 5th Morphology criteria are similar, as they are based on the same strict principles, similar guidelines and reference publications. As the WHO 4th and 5th criteria are similar, we did not conduct any additional study for the WHO 5th morphology algorithm and stayed with the same developed for the WHO 4th. Only the reference value was changed in the V-Sperm.

Answer 58

difference between WHO 4th and 5th