HCV lecture 4 therapy old and new Flashcards
what does IFN-alpha do
activates cellular interferon mediated antiviral responses.
what does pegylated IFN-alpha / Albuferon do
used in combination has a longer half life
why is ribavirin used in combination therapy aswell
it has a broad antiviral range.
what did the interferon regime treatment consist of
weekly 180 mg PEG-IFN or Albuferon by injection. daily ribavarin 800-1400 mg orally given/
what is ribavarin
a guanosine analogue incorporated into the viral genome RNA by NS5B. I is mutagenic.
name some disadvantages of IFN based treatment
responds differently to different genotypes. alot of relapses after therapy. has side effects. expensive.
what do DAAs target in the HCV life cycle
translation and polyprotein processing and RNA replication.
how can resistance develop
NS5B polymerase is error prone, selection pressure from treatment, random mutations, pre existing mutants.
what are the HCV quasi-species
when the error prone polymerase creates a mixture of genomes the fittest ones best replicate theses are the quasi-species.
what do telaprevir, boceprevir and simeprevir target
the protease NS3/4A
what are glecaprevir and voxilaprevir
they are 3rd generation inhibitors of HCV protease
what does NS3/4A block
the induction of IFN-beta expression
where does the resistnace substitutions map for NS5A inhibtors
amphipathic helix and domain 1
what are daclatasvir and velpatasvir
NS5A inhibitors
what do sofosbuvir and dasabuvir target
NS5B RNA polymerase
