head start (pg 4 - 30) ALSO DO HEART REVISION Flashcards
(171 cards)
what is metabolism?
in a living cell thousands of biochemical reactions take place every single second. The sum of these reactions is called metabolisms
what is a metabolic pathway?
a single chain of biochemical reactions that take place in a living cell
what would happen to the biochemical reactions inside a body if they didn’t have enzymes?
these reactions would take place very slowly at normal body temperature
what are enzymes?
enzymes are biological catalysts that increase the rate of reactions
how do enzymes act as catalysts?
a reaction that needs a high activation energy can’t start at a low temperature of 37 degrees (i.e. body temperature). Enzymes reduce the activation energy
what type of proteins are enzymes?
globular proteins (because they’re roughly spherical)
what does the order of amino acids in an enzyme determine?
its structure, and so how it works
give 2 examples of what enzymes help with
enzymes can be involved in breaking down molecules or building molecules. For example:
- Digestive enzymes are important in the digestive system, where they help to break down food into smaller molecules, e.g. carbohydrases break down carbohydrates
- enzymes involved in DNA replication help to build molecules, e.g. DNA polymerase
what is the substance that’s acted upon by an enzyme called?
the substrate
what is the active site?
the region on the surface of the enzyme molecule where a substrate molecule can attach itself. It’s where the catalysed reaction takes place
how quickly do enzymes break down substrate molecules?
almost as soon as the enzyme-substrate complex has formed, the products of the reaction are released and the enzyme is ready to accept another substrate molecule
describe the effect of temperature on enzyme activity
as temperature increases, enzyme reactions become faster, because the molecules have more energy. However, at high temperatures the atoms of the enzyme molecule vibrate more rapidly and break the weak bonds that hold the tertiary structure together. The shape of the active site changes and the substrate can no longer fit in. The enzyme is said to be denatured
describe the effect of pH on enzyme activity
acids and alkalis can denature enzymes. Hydrogen ions (H+) in acids and hydroxyl ions (OH-) in alkalis disrupt the weak bonds and change the shape of the active site
what is the name of the OH- alkali ion?
hydroxyl
what is the function of the cytoplasm of the cell?
it contains enzymes that speed up biochemical reactions
what does the vacuole contain?
cell sap, a weak solution of sugar and salts
how big are prokaryotic cells compared to eukaryotic cells?
prokaryotes like bacteria are roughly a tenth the size of eukaryotic cells
name 3 organelles that prokaryotic cells don’t have
- nucleus
- mitochondria
- chloroplasts
what is the largest magnification a light microscope can reach? what does this allow you to see?
1500x (this allows you to see individual animal and plant cells along with the organelles inside them)
which parts of a cell can you see with a light microscope?
- If the cells have been stained you can see the dark-coloured nucleus surrounded by lighter-coloured cytoplasm
- tiny mitochondria and the black line of the cell membrane are also visible
- in plant cells, the cell wall, chloroplasts and the vacuole can be seen
give a different name for a light microscope
an optical microscope
what is the part of the microscope you look down called?
the eyepiece
what are the names of the two different knobs on the microscope, and how are they different?
the larger knob (the coarse adjustment knob) is to bring the specimen roughly into focus. The smaller knob (usually lower down than the coarse adjustment knob) is called the fine adjustment knob and is to finely tune the focus.
what is the name of the area where you put your microscope slide?
the stage