HeLa Cells Exposed to Cisplatin and Mitomycin C Flashcards Preview

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Flashcards in HeLa Cells Exposed to Cisplatin and Mitomycin C Deck (9):
1

E6

inactivates p53

2

E7

inactivates pRB- prefers the active form of pRB

3

E6 and E7 together effect

prevent apoptosis and promote cell cycle progression

4

E6 and E7 together effect

prevent apoptosis and promote cell cycle progression

5

Inactivation of p53 by E6

E6 and E6AP make a complex that acts as a ligase for p53

6

Cisplatin

alkalating agent that prevents replication by forming cross links intrastandedly or interstrandedly of the DNA

7

Mitomycin C

antibiotic
inhibits DNA synthesis by forming cross links between the complementary strands; therefore, the strands can't separate during replication
acts in late G1/early S

8

Stages of apoptosis

cell shrinkage --> nuclear condensation --> further condensation --> membrane blebbing, apoptotic bodies, and fragmentation of DNA

9

EC50

the effective concentration of a drug that gives half-maximal response (death)