Hema

Question 1

Specimen collected in sodium citrate tubes (BLUE TOP) must be
processed within __ after blood collection

Answer 1

4 hours

Question 2

What are the two machines used in lab

Answer 2

-UniCel DxH 800 (Hematology analyzer machine)

→ STS Compact Max (Coagulation analayzer)

Question 3

mcv normal range

Answer 3

80-96 femtoliters per cell;

SIZE

Question 4

Average amount of hemoglobin per single RBC

picograms/ cell

Answer 4

mch

Question 5

Average concentration of Hb per unit volume of RBCs; Hemoglobin per hematocrit (g/dL)

Answer 5

MCHC

Question 6

If ALL blast lineage cells observed

− Usually observed in adult patients

Answer 6

Chronic myelogenous leukemia (CML)

Question 7

Ideal ratio sodium citrate anticoag;
if blood insufficient- slower clotting
if blood excess-faster clotting

Answer 7

1-part anticoagulant: 9 parts blood

Question 8

Which two tests are measured using the STS Compact Max

Answer 8

Prothrombin Time (PT) Extrinsic pathway and
Partial Thromboplastin Time  (ptt )Intrinsic pathway

Question 9

Retic count uses ___; where living cells take up ___

Answer 9

supravital staining;

Brilliant Cresyl Blue;

Question 10

___protein C or protein S level or activity= result in excessive
or inappropriate blood clottinG

Answer 10

Low;
 Indicative of 
DIC
Deep venous thrombosis
Pulmonary embolism

Question 11

Two modes of Beckman coulter

Answer 11

Casette-mode

Single tube mode (microtainer)

Question 12

Principle of Beckman coulte

Answer 12

Electrolytes in diluent dtected produce CURRENT; struck by light source= CHARGED VOLTAGE or PULSE

Question 13

Flow cytometry is for

Answer 13

WBC diff count;

by Light scatter and fluorochrome

Question 14

X axis of flow cyto;

Y axis

Answer 14

peroxidase uptake;

size of cell

Question 15

Gran vs Agranulocytes based on uptake of peroxidase

Answer 15

§ Granulocytes: High uptake

§ Agranulocytes: Low uptake

Question 16

Rightmost in scatterplot means;

Highest means

Answer 16

highest uptake;

biggest size

Question 17

Colors in scatterplot

Answer 17

Neutrophil: Pink; Lymphocyte: blue; Monocyte: Green;
Eosinophil: Orange; Basophil white;
RBC: Red

Question 18

What principle is used by the Stago Compact Max machine in Hema Lab

Answer 18

Mechanical;
small metal beads;
machine add activator and CaCl to start clotting;

time stops when metal STOPS MOVING;

No interference and can still work even if samples are hemolyzed