Hematological Determinations

Question 1

1 g of Hemoglobin is qual to how many mL of oxygen?

Answer 1

1 g Hb = 1.34 mL of Oxygen

Question 2

Reagent use for Acid Hematin

Answer 2

0.1 Normal HCl

Question 3

reagent use for alkali hematin

Answer 3

0.1 normal NaOH

Question 4

enumerate the 3 direct visual colorimetric method

Answer 4

acid hematin, alkali hematin, & tallquist method

Question 5

Considered the quickest method of assessing
a patient’s hemoglobin level

Answer 5

Tallquist method

Question 6

Direct method done by dropping blood in a special type of paper and compare w/ the colored chart

Answer 6

tallquist method

Question 7

most reliable and recommended method for
Hgb measurement

Answer 7

Indirect (Cyanmethemohlobin)

Question 8

Cyanmethemoglobin is able to measure all forms of Hgb except

Answer 8

Sulfhemoglobin

Question 9

Colorometric method that is not affected by coagulation

Answer 9

Cyanmethemoglobin

Question 10

reagent use for cyanmethemoglobin method

Answer 10

Detergent-modified Drabkin’s reagent

Question 11

purpose of detergent in cyanmethemoglobin method

Answer 11

enhance lysis of RBC; decreased turbidity

Question 12

component of drabkin’s reagent that Converts hemoglobin to hemiglobin

Answer 12

potassium ferricyanide

Question 13

components of drabkin;s reagent that

Answer 13

Adds the cyanide ions to producehemiglobin cyanide(HiCN) w/ci s measured at a spectra of 540nm

Question 14

Shortens the time needed for
complete conversion of Hb to HiCN

Answer 14

Dihydrogen potassium phosphate

Question 15

Considered the one w/ greatest accuracy because it estimates oxygen combining power of the blood

Answer 15

Gasometric (Van Slyke Capacity Method)

Question 16

Impractical because of using big instrument

Answer 16

Gasometric

Question 17

DRAWBACK: Affected by myeloma proteins, abnormal globulins, radiographic contrast
media

Answer 17

Copper Sulfate Method

Question 18

Use to screen blood donors before

Answer 18

Copper Sulfate Method

Question 19

SG of copper sulfate =

Answer 19

1.053

Question 20

SG of copper sulfate = to how much of Hemoglobin?

Answer 20

SG of copper sulfate = 12.3 g/dL of Hgb

Question 21

1 g of Hb is equal to how many content of iron?

Answer 21

3.47 mg but 3.35 if according to Alba

Question 22

Measurement of carboxyhemoglobin
concentration in the blood

Answer 22

Katayama’s test

Question 23

reagent use in katayama’s test

Answer 23

ammonium sulfide

Question 24

Positive COHgb =

Answer 24

rose red color

Question 25

PACKED CELL VOLUME (PCV); Measures the concentration of RBCs in a given volume of whole blood in a capillary tube

Answer 25

Hematocrit

Question 26

clarifying various degrees of anemia & monitoring acute hemorrhagic conditions

Answer 26

Hematocrit

Question 27

hematocrit is one of the basis for diagnosing ------

Answer 27

polycythemia

Question 28

Macromethod that used sodium oxalate as the main reagent

Answer 28

Van Allen Sanford-Margath Haden Mnemonic: VaSaHa

Question 29

reagent or main component use for bray macromethod

Answer 29

Heparin

Question 30

Speed of Centrifugation of macromethod

Answer 30

2000-2300 g (30 mins)

Question 31

tube Use for Hct & ESR

Answer 31

Wintrobe Tube

Question 32

length of wintrobe tube

Answer 32

11.5 cm

Question 33

bore size of wintrobe tube

Answer 33

3 mm

Question 34

Wintrobe tube's Calibration mark (ESR):

Answer 34

0-100 (white mark)

Question 35

Wintrobe tube's Calibration mark (Hct)

Answer 35

10-0 (red mark)

Question 36

Most common method because of using less blood; less time consume; better packing ofcells

Answer 36

Micromethod (Adam's method)

Question 37

speed of centrifugation of micromethod

Answer 37

10,000-15,000 g (5 mins)

Question 38

Microhematocrit tube length

Answer 38

70-75 mm or 7 cm

Question 39

microhematocrit tube bore size

Answer 39

1 mm

Question 40

Length of Sealing Clay:

Answer 40

4-6 mm

Question 41

Source of errors: overcentrifugation

Answer 41

falsely decrease

Question 42

Source of errors: inadequate centrifugation

Answer 42

falsely increase

Question 43

Source of errors: Improper sealing

Answer 43

falsely decrease

Question 44

Source of errors: Short draw

Answer 44

Falsely decrease

Question 45

Source of errors: Introduction of interstitial fluid from a skin puncture

Answer 45

falsely decrease

Question 46

best diluting fluid for RBC count

Answer 46

Dacie's

Question 47

Dilution factor of RBC count

Answer 47

200

Question 48

Hgb is how many grams higher in male?

Answer 48

1-2 g/dL

Question 49

Components of Turk's diluting fluid

Answer 49

Gentian violet & Glacial acetic acid & hydrogen peroxide

Question 50

characteristic of WBC diluting fluid

Answer 50

hypotonic

Question 51

Dilution ratio of WBC

Answer 51

20

Question 52

Phase contrast microscopy method  Uses 1 % ammoniumoxalate ADVANTAGE: increase refractility; considered as a reference method for platelet count

Answer 52

Brecker-Cronkite Method

Question 53

3 components of Rees-Ecker diluting fluid

Answer 53

sodium citrate, brilliant cresyl blue, and formalin

Question 54

Fragments of leukocyte cytoplasm that are sometimes numerous in leukemias may falsely _____ the count

Answer 54

elevate; increase

Question 55

These can occur if platelets adhere to neutrophils agglutinins, spontaneous aggregation, or incipient clotting due to faulty blood collection.

Answer 55

Plalelet satellism

Question 56

10 squares arranged in 2 horizontal rows with 5 columns each; each square is further subdivided into 16 smaller squares

Answer 56

Speir's Levy

Question 57

A red cell measurement that quantitates cellular volume heterogeneity reflecting the range of red cell sizes w/in a sample  Useful in early classification of anemia

Answer 57

Red Cell Distribution Width (RDW)

Question 58

Applies only to samples that have a normocytic normochromic red blood cells

Answer 58

Rule of Three

Question 59

For Bone Maroow aspirates crush prep

Answer 59

Cover Glass Smear

Question 60

produces a uniform blood film, in which all cells are separated (a monolayer) and randomly distributed

Answer 60

Spinner Method