Hematology

Question 1

Do we perform differentials on Buffy coat?

Answer 1

No

Question 2

What is the heparin tube used for in hematology?

Answer 2

osmotic fragility, LAP score

Question 3

What is the ME ratio?

Answer 3

2:1 to 5:1 - lymphs not included

Question 4

What is the lifespan of platelets?

Answer 4

5-10 days

Question 5

How many platelets are there per field?

Answer 5

8-10

Question 6

What is Hbg F made out of?

Answer 6

2 alpha and 2 gamma

Question 7

What does Hbg F change to?

Answer 7

Hb A

Question 8

What is Hbg A made out of?

Answer 8

2 alpha and 2 beta

Question 9

What is Hbg A2 made out of?

Answer 9

2 alpha and 2 delta

Question 10

What is carboxyhemoglobin?

Answer 10

Carbon monoxide bound with hemoglobin

Question 11

What is methhemoglobin?

Answer 11

When iron goes from ferrous to ferric state

Question 12

Is carboxyhemoglobin/methemoglobin reversible?

Answer 12

Yes

Question 13

What is the Drabkin’s solution used for?

Answer 13

Measure hemoglobin colormetrically

Question 14

When do we correct WBC’s with the formula?

Answer 14

> 10 nRBC

Question 15

What is the correction formula for WBCs?

Answer 15

(#WBC counted/#WBC + nRBC counted) x WBC of the instrument

Question 16

What is the DF for a manual RBC count?

Answer 16

200

Question 17

When do we use a Neubauer hemocytometer?

Answer 17

Count high # of WBCs and PLTs (depth: 0.1 mm)

Question 18

When do we use the Fuchs hemocytometer?

Answer 18

Count low # eosinophils and body fluids

Question 19

What makes up the Wrights stain?

Answer 19

methylene blue, eosin, methanol, giemsa

Question 20

What stain is required for a bone marrow sample?

Answer 20

Perls Prussian Blue for hemosiderin (form iron takes in the bone marrow)

Question 21

Where is the main storage site for iron?

Answer 21

Bone marrow

Question 22

What is the secondary storage site for iron?

Answer 22

liver

Question 23

What stain is used for reticulocytes?

Answer 23

Supravital stain (ex: new methylene blue)

Question 24

What objective do we use for retics?

Answer 24

oil immersion

Question 25

Which of the following actions may cause a body fluid cytosine preparation to have a cell button that is too thick? a. Sample vortexes prior to slide preparation b. Sample stored at 22C for many hours c. Sample not mixed prior to slide preparation d. Sample diluted prior to slide preparation

Answer 25

C

Question 26

Which of the following morphological findings correlate with the chronic blood loss?

Answer 26

Micro/hypo

Question 27

Which of the following anemias correlates with the given results? RBC 3.30 Hgb 110 g/L HCT 0.298 a. Aplastic b. Hereditary spherocytosis c. Hereditary elliptocytosis d. Thalassemia

Answer 27

B

Question 28

Aspirin blocks plt plug formation by interfering with what process? A. Secretion B. Shape change C. Aggregation D. Adhesion

Answer 28

C

Question 29

Heparin antagonist that will remove heparin from blood samples collected from patients on heparin therapy:

Answer 29

Protamine sulphate

Question 30

Which enzyme is responsible for fibrinolysis in vivo? A. Coagulase B. Plasmin C. Fibrinolase D. Thrombin

Answer 30

B

Question 31

What is defective glycoprotein IIb/IIIa associated with?

Answer 31

Glanzmann thrombasthenia

Question 32

In polycythemia vera, the platelet count is: A. Elevated B. Decreased C. Normal D. Variable

Answer 32

A

Question 33

What causes a decrease in osmotic fragility?

Answer 33

Sickle cell anemia due to increased SA: V ratio (less fragile and more deformable)

Question 34

Used to confirm diagnosis of activated protein C resistance:

Answer 34

Factor V Leiden mutation assay