Hematology Flashcards
(30 cards)
What type of dye is used in a reticulocyte count?
Methylene Blue
A reticulocyte count is a measurement of what kind of response?
A regenerative response
How are reticulocyte a different from normal rbc?
They have dark spots or lines in them .
What is the ratio of reticulocyte to rbc?
reticulocytes/1000 rbc
Cats have how many types of reticulocytes?
2
What are the two types of cat reticulocytes?
Punctate and Aggregate
Which of the cat reticulocytes are the ones you count in a reticulocyte count?
Only the aggregate ones.
Describe punctate reticulocytes.
They have 2-8 basophilic stipples.
Describe aggregate reticulocytes.
They have basophilic lines - more like the typical reticulocytes.
What does the CBC machine lyses?
Everything without a nucleus. (Mature RBC, platelets)
When will a CBC machine count a RBC as a WBC?
If the RBC has a nucleus, it is not lyses, therefore it is counted as a WBC (artificially elevating WBC count).
When would we know if a blood sample has N-RBCs?
When a differential is performed.
How are N-RBCs seen on a differential recorded?
N-RBC/100 WBC
How must a differential be corrected if N-RBCs are found on it?
Observed WBC count x 100 ——————————— 100 + N-RBC
When do fibrinogen levels increase?
During inflammation.
What is something that can be done in order to measure fibrinogen levels?
By heating plasma to 56C.
What happens to plasma at 56C?
Fibrinogen pellets out, but other plasma proteins stay suspended.
What the procedure for testing fibrinogen?
- Spin two hematocrit tubes.
- Measure TP with one of the hematocrit tubes.
- Incubate the other hematocrit in a 56C water bath for 5 minutes.
- Respin heated hematocrit and measure TP.
- Figure the fibrinogen level by subtracting the second TP from the first TP. Record as mg/dl.
What is crossmatching used for?
To determine compatibility for blood transfusions.
What indicates incompatibility?
Agglutination (RBC clumping)
How can you blood type cats?
Via cards similar to humans.
How many blood types do dogs have?
11 types of blood.
What is the difference between a major and minor crossmatch?
Major: Recipient serum is tested against donor packed cells to determine if the recipient has preformed antibodies against any antigens on the donor’s cells.
Minor: Recipient RBCs are tested against donor serum to detect donor antibodies directed against patient’s antigens.
What causes a hematoma?
Needle has passed through vein.
Bevel of needle is only partially in vein.
Insufficient pressure on venipuncture site after needle is withdrawn.
