HEMOGLOBIN DETERMINATION (ACID HEMATIN & CYANMETHEMOGLOBIN)

Question 1

It is the red iron bearing protein contained within the erythrocytes in normal blood

Answer 1

Hemoglobin

Question 2

What is the function of hemoglobin?

Answer 2

Carry oxygen & carbon dioxide to facilitate gas exchange

Question 3

Hemoglobin is composed of

Answer 3

• heme molecules
• globin chains

Question 4

Represents colored component

Answer 4

Heme

Question 5

It is a complex protein

Answer 5

Globin

Question 6

What is the molecular weight of one Hb molecule?

Answer 6

64,458 daltons

Question 7

What are the 3 factors that affected hemoglobin?

Answer 7

• age
• sex
• altitude of the locality (the higher the place, the higher your hemoglobin become)

Question 8

Usually used in blood bank

Answer 8

Copper sulfate or specific gravity method

Question 9

What is the reagent of acid hematin?

Answer 9

0.1 normal hypochloric acid

Question 10

Reagent of alkali hematin

Answer 10

Sodium hydroxide

Question 11

Best metgod for measuring plasma hemoglobin

Answer 11

Oxyhemoglobin method

Question 12

Reagent for cyanmethemoglobin

Answer 12

Drabkin’s reagent + 20 uL blood

Question 13

Presence of free hemoglobin in the plasma confined with the RBCs the hemoglobin is release into the plasma

Answer 13

Hemoglobinemia

Question 14

Decrease in anemia

Answer 14

Oligochromia

Question 15

Hemoglobin Reference ranges for adult male

Answer 15

13.0-17.0 gm/dl

Question 16

Hemoglobin Reference ranges for adult female (non-pregnant)

Answer 16

12.0-15.0 gm/dl

Question 17

Hemoglobin Reference ranges for adult female (pregnant)

Answer 17

11.0-14.0

Question 18

Critical values interpretation for < 7 gm/dl

Answer 18

Severe anemia

Question 19

Critical values interpretation for 20 gm/dl

Answer 19

Hyperviscosity

Question 20

All hemoglobin derivaties except _______ are measured with their conversion to cyanmethemoglobin

Answer 20

sulfhemoglobin

Question 21

If the charge of iron within the
hemoglobin is converted from 2+ to 3+
that hemoglobin is already called ________

Answer 21

Methemoglobin

Question 22

The one who convert hemoglobin to methemoglobin

Answer 22

Ferricyanide

Question 23

Color of acid hematin

Answer 23

Brownish yellow color

Question 24

Drabkin’s reagent consist of

Answer 24

• ferricyanide
• potassium cyanide