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HF1 Flashcards

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1
Q

lyseren voor verkrijgen van DNA

A

humaan klein weefsel
grote weefsels
boed
bacteriën

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2
Q

fenol-chloroform

A

isoleren van DNA

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3
Q

cesium-chloride densiteit chromatografie

A

isoleren van DNA

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4
Q

alcohol precipitate

A

concentreren van DNA

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5
Q

concentrator en Speedvac

A

concentreren van DNA

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6
Q

dipstick

A

kwantificatie DNA

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7
Q

OD260

A

kwantificatie DNA

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8
Q

enzymatische assay

A

kwantificatie DNA

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9
Q

kwantificatie DNA

A

OD, dipstick, enzymatische assay, gelkwantificering

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10
Q

manipuleren en kloneren van DNA

A

restrictie digestie, aspecifieke fragmentatie, uiteinde invullen, uiteinden afknabbelen, merken van DNA, scheiding op gel, vector, ligeren, TA-cloneren, kloneren met recombinatie

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11
Q

primaire sequentie bepaling

A

chemische en Sanger

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12
Q

NGS

A

454/Roche, Illumina, nanopore, ion/proton sequencing, PacBio (ABI-SOLID)

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13
Q

Southernblot

A

genidentificatie DNA

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14
Q

RFLP

A

genidentificatie DNA

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15
Q

ARMS

A

genidentificatie DNA

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16
Q

melting analysis

A

genidentificatie DNA

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17
Q

comparatieve genomic hybridization

A

genidentificatie DNA

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18
Q

oligonucliotiden assay

A

genidentificatie DNA

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19
Q

Q-RT-PCR

A

genidentificatie DNA

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20
Q

genidentificatie DNA

A

southernblot, RFLP, ARMS, melting analysis, cooperative genomes, oligonucl assay, Q-RT-PCR

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21
Q

quanidine isothiocyanaat/fenol

A

RNA bereiding

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22
Q

ionenuitwisselingschromatigrafie

A

isoleren van DNA + Bereiding RNA

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23
Q

RNA bereiding

A

quanidine isothiocyanaat/fenol en ionenuitwisselingschromatigrafie

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24
Q

northernblot

A

transcriptieidentificatie RNA

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25
RT-PCR
transcriptieidentificatie RNA
26
transcriptie identificatie
northern blot, RT-PCR, in situ hyridisatie
27
Detectie in PCR
Taqman, SYBRGreen, Molecular beacon, FRET
28
DNA microarray
nylon, glas, fotolythografisch | +kleur manieren!!
29
single cell transcription
DROP-seq | CITE-seq
30
DROP-seq
single cell transcription RNA
31
CITE-seq
single cell transcription RNA
32
spatial transcriptoom
geo-seq, in situ seq
33
aanmaak van eiwitten
chemisch (f-moc), enzymatisch (wheat germ), in vivo, Ab
34
uitzouten
scheiden van eiwitten
35
gel-elektroforese op eiwitten
scheiden van eiwitten
36
chromatografie op eiwitten
scheiden van eiwitten
37
scheiden van eiwitten
uitzouten, gel-elektroforese, chromatografie
38
concentratiebepaling van eiwitten
UV, colorimetrisch, fluometrisch
39
colorimetrische testen
concentratie bepaling eiwitten
40
eiwitidentificatie
kleuren in gel, ELISA, RIA, Edman, westernblot, MS/MS, MALDI, immunohistochemie, delfia
41
ELISA
eiwitidentificatie
42
RIA
eiwitidentificatie
43
Delfia
eiwitidentificatie
44
westernblot
eiwitidentificatie
45
Edman
eiwitidentificatie
46
exploratieve proteomics
bottom-up: 2D-gel, chromatografie
47
targetted proteomics
MS-based, SOMAmer
48
differentiele proteomics
2D-DIGE, SILAC, ICAT, ITRAQ
49
NGP
portie fingerprint fluo, nanopore, e-tunneling
50
spatial proteomics
MILAN, IMC, MS-imaging
51
single cell eiwit analyse
single cell westerns, CyTOF
52
CyTOF
single cell eiwit analyse
53
eiwit-eiwit interactie
yeast-2-hybrid, MAPPIT, affinity pull down, FRET, RIME, proximity ligation test, array based, immunoprecipitatie, SPR, faag
54
yeast-2-hybrid
eiwit-eiwit interactie
55
MAPPIT
eiwit-eiwit interactie
56
affinity pull down
eiwit-eiwit interactie
57
RIME
eiwit-eiwit interactie
58
SPR
eiwit-eiwit interactie
59
proximity ligation test
eiwit-eiwit interactie
60
analyse gentranscriptie
run-on, promotor-reporter, DNase foot printing,DNAse seq, FAIRE, EMSA, affinity chromatography, chip
61
chip
analyse gentranscriptie
62
FAIRE
analyse gentranscriptie
63
EMSA
analyse gentranscriptie
64
DNAse footprinting
analyse gentranscriptie

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