Histocompatibility

Question 1

When should a lab report HLA typing on a deceased kidney, kidney-pancreas, pancreas, or pancreas islet donor?

Answer 1

Prior to organ offers

Question 2

When should a lab report HLA typing on a deceased heart, heart-lung, or lung donor?

Answer 2

Prior to final acceptance, if required by the transplant program

Question 3

When should a lab report HLA typing on a deceased liver donor?

Answer 3

Within the time period specified by the transplant program

Question 4

If a critical discrepancy in HLA typing is found post-procurement what must the OPO do?

Answer 4

Notify and provide
supporting documentation to all accepting transplant programs within 24 hours

Question 5

What must a laboratory do in a HLA antibody screening? (2 components)

Answer 5

1.Report anti-HLA antibodies identified to the candidate’s requesting provider
2. Use at least one solid phase immunoassay using purified HLA molecules

Question 6

General crossmatching requirements (4 listed requirements)

Answer 6

1.Perform a crossmatch according to the terms specified in the written agreement between
the laboratory and the OPO or transplant program
2. Crossmatch potential donor T lymphocytes to identify class 1 anti-HLA antibodies
3. Crossmatch potential donor B lymphocytes to identify class 1 and class 2 anti-HLA antibodies
4. Use a crossmatch technique with increased sensitivity

Question 7

How long should a lab preserve specimens from a deceased donor for subsequent histocompatibility testing after transplant?

Answer 7

5 years!

Question 8

Which organ’s allocation may be denied based on a discovery of cytomegalovirus (CMV)?

Answer 8

Intestine