Histological Techniques Flashcards
(41 cards)
What is the purpose for Histological Techniques?
-Storing Tissue Samples
What is Formaldehyde?
-Preservative
How is preserved tissue sliced?
-Thinly
How is a tissue ‘impregnated’ with wax?
-Dehydrated first then wax added
Word used to describe changes to tissue?
-Artifacts
What happens when dye is added to a tissue?
- Binds to molecules
- Adding dye is called ‘Stains’
Name the two types for Dye. (H&E)
- Haematoxylin
- Eoisn
- Have affinity for opposite pH
What happens when Haematoxylin dye is added?
- It is alkaline and has affinity for acidic molecules
- Turns purple/blue
What happens when Eosin is added?
- It is acidic and has affinity for basic (alkaline) molecules
- Turns pink
Name X4 Basic Tissue Types
-Epithelial
-Connective
-Nervous
-Muscle
(MENC)
What are the features of Epithelial Tissue?
- Adhesions-strong bands between tissues and organs
- Basal lamina- basement membrane
- Basal Surface
What are the functions of Epithelial Tissue?
- Physical Barrier
- Chemical Barrier
- Absorption
- Secretion
- Containment
- Locomotion-movement
What features can classify epithelial tissue?
- Shape
- Surface
- No. of layers
- Specialsed cells
What are X3 shapes of Epithelial Tissue
- Squamous
- Cuboidal
- Columnar
What are glands made of?
-Epithelial Tissue
Name X2 Types of Glands
-Endocrine and Exocrine
Function of Endocrine Glands?
-Product is secreted towards Basal end of cell (bottom(
Function of Exocrine Glands?
-Product is secreted towards the Apical end of cell (top)
Name X3 Types of Connective Tissue
-Soft
-Hard
-Blood and Lymph
(determined by no. cells packed)
Name types of Soft Tissue?
-Tendons and Ligaments
Name types of Hard Tissue
- Hyland Cartilage
- Bone
What does Blood and Lymph Tissue form?
-Specialised cells
Name X4 key features of Connective Tissue
- Extracellular Matrix
- Fibres-collagen etc
- Tissue fluid
- Ground Substance
What are Osteocytes?
-cells of the bone