Histology

Question 1

Histology

Answer 1

The study of structure and function of individual cells and organ systems

Question 2

Histotechnology

Answer 2

Techniques used to prepare TISSUES for microscopic examination and diagnosis

Question 3

Histopathology

Answer 3

The study of diseased tissues

Question 4

Histochemistry

Answer 4

Marking the location of known substance within the cellular tissues/structures by reacting substances with chemical solutions, i.e. special stains

Question 5

Immunohistochemistry

Answer 5

Antigen/antibody reactions are used to achieve histochemistry

Question 6

Cytotechnology

Answer 6

Technology used to prepare CELLS exploited or aspirated for microscopic examination

Question 7

What (5) questions do specimens answer about the patient

Answer 7

Is the specimen normal
Is the lesion Bennie or malignant
What is the patients prognosis
Anything unexpected detected
Is there a correlation with other findings

Question 8

Prognosis

Answer 8

Predict a probable outcome based on patient condition against the usual course of disease in similar circumstances

Question 9

Common sources of submitted tissues

Answer 9

Autopsy, surgical tissue
Whole or partial organ
Excited tumor or cyst
Curettage
Amputation
Products of conception
Biopsy or exfoliated cells
Requires by law

Question 10

Exemptions of tissues not required to send to lab

Answer 10

Teeth, varicose veins, hernias, foreskin, children’s tonsils, normal placentas

Question 11

Specimen prep: sectional method

Answer 11

Cuts section into thin, translucent slices of tissue (4-6um)
Preserves tissue pattern where cell relationship is demonstrated

Question 12

Specimen prep: touch preparation

Answer 12

Cut side of fresh tissue is touched to slide and imprints specimens architectural arrangements
May be used to detect malignancy or microorganisms

Question 13

Specimen prep: Fresh cells

Answer 13

Cells suspended in their own fluids
Examined directly, under a microscope, in a drop of fluid

Question 14

Specimen prep: Dissociation

Answer 14

Material “teased” apart to break up pattern and allow individual cells to be examined
Not routinely done- may have application in research

Question 15

Specimen prep: Fluid spread on slide

Answer 15

Cells “fixed” to slide to preserve appearance, stained, mounted, examined (cytology, hematology)

Question 16

Tissue examination: Gross examination

Answer 16

Macroscopic evaluation
Initial examination and subsequent description of all specimens by a pathologist/technologist

Question 17

Tissue examination: Brightfeild Microscopy

Answer 17

Routinely used to examine tissue sections

Question 18

Tissue examination: Polarizing microscope

Answer 18

Used to identify certain materials

Question 19

Tissue examination: fluorescent microscope

Answer 19

Useful in immunohistochemistry where antigens found in tissues are detected

Question 20

Tissue examination: Electron microscope

Answer 20

Approximately 200,000x magnification
Usually for research and in study of viruses