Histology Flashcards
(7 cards)
1
Q
What can uneven illumination result in while using a microscope?
A
Artefacts, such as shadowing and glare
2
Q
What can be done to avoid artefacts?
A
Use Kohler illumination
3
Q
What stains are often described as a “general tissue stain”
A
haematoxylin and eosin
4
Q
What colour and where does eosin stain?
A
Cytoplasm and connective tissue in shades of pink/red
5
Q
What colour and where does haematoxylin stain?
A
Cell nuclei in blue/purple
6
Q
What mixtures are used to dewax samples?
A
XTF, absolute alcohol, 70% alcohol and water
7
Q
What mixtures are used to stain the samples?
A
haematoxylin, water, 0.5% v/v acid alcohol, Scott’s tap water substitute, eosin, absolute alcohol and XTF
