Erythrocytes constitute approximately what percentage of whole blood (i.e., hematocrit) in healthy men and healthy women? How many erythrocytes are there in a ml of blood?

Erythrocytes constitute approximately 37 to 49% of whole blood. There are 4.5 x 10^9 – 6.4 x 10^9 erythrocytes in a mL of blood

Histology Flashcards by Julia Moulton

Erythrocytes constitute approximately what percentage of whole blood (i.e., hematocrit) in healthy men and healthy women?
How many erythrocytes are there in a ml of blood?

Erythrocytes constitute approximately 37 to 49% of whole blood.
There are 4.5 x 10^9 – 6.4 x 10^9 erythrocytes in a mL of blood

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Platelet

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What granulocytes are found in the peripheral blood?

Neutrophils, eosinophils, basophils

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What agranulocytes are found in the peripheral blood?

Monocytes, lymphocyte

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Neutrophil

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Neutrophils constitute what percentage of circulating leukocytes in a normal blood smear?

They make up 48-76% of circulating leukocytes.

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What is the function of neutrophils?

Neutrophils contain granules (such as primary – myeloperoxidase, elastase, antibacterial proteins, secondary – lysozyme, tertiary – alkaline phosphatase) that are responsible for fighting off local pathogens in tissue. They are fast acting and often are the first responder in an infection.

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Eosinophil

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Eosinophils constitute what percentage of circulating leukocytes in a normal blood smear?

Less than 5%.

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What are the functions of eosinophils?

Eosinophils can seek out or dampen the inflammatory reactions and are antiparasitic.

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Basophil

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Basophils constitute what percentage of circulating leukocytes in a normal blood smear?

0-2% of leukocytes.

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What is the primary function of basophils?

Basophils incite inflammatory responses. They bind to circulating IgE via IgE receptor on their cell surface and the IgE then binds to antigens, resulting in activation of the basophil and degranulation. Basophil granules contain histamine, which plays a big role in initiating an inflammatory response.

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What resident connective tissue cell functions similarly to basophils, and shares a common progenitor cell?

Mast cells

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Monocyte

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Monocyte

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Monocytes constitute what percentage of circulating leukocytes in a normal blood smear?

2.5-8.5%

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What is the function of monocytes?

Become macrophages in tissue, engulf foreign invaders, dead tissue, tumor cells.

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Small lymphocyte

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Large lymphocyte

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Lymphocytes constitute what percentage of circulating leukocytes in a normal blood smear?

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18-41%

Name the three main classes of lymphocytes.

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B cells, T cells, NK cells

Describe the morphologic differences between the erythrocytes seen in iron deficiency anemia and those in the normal blood smear.

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The RBCs are smaller and have a larger central pallor, with the staining presenting mainly on the outline of the cell. The shape is consistent.

What is the role of iron in red blood cells?

Necessary co-factor in the transformation of proto-phorphyrin IX into heme

What other nutrients are necessary for proper RBC production?

Vitamin b12 and Folate

Why does iron deficiency lead to anemia?

It limits the formation of heme, which limits hemoglobin synthesis and effects the ability to carry oxygen in circulation.

Cells of the erythroid lineage have [A] nuclei at all stages where nuclei are present and [B] cytoplasm. As these cells mature, the cell and nucleus become [C], the nucleus becomes more [D], and the cytoplasm turns from [E] to [F] to [G] to red. The final stage is the mature erythrocyte, which has no nucleus and is small and red.

[A] round [B] non- granulated [C] smaller [D] heterochromatic [E] pale blue [F] dark blue [G] gray

Cells of the myeloid lineage start as large cells with [A] nuclei and a few small [B] granules, progressing to cells with an [C] nucleus and accumulations of [D] in the cytoplasm. The [D] may be [E] . Mature granulocytes (neutrophils, eosinophils, basophils) are present and can be identified by their characteristically [F] nuclei, and cytoplasm completely filled with specific [D].

[A] round [B] azurophilic [C] ovoid or indented [D] granules [E] neutrophilic, eosinophilic, or basophilic [F] lobed

What is the cause of the basophilic (blue) material in the cytoplasm during early stages of erythroid differentiation?

During maturation, the overall size of the erythroid precursor cell reduces with the cytoplasmic to nucleus (C:N) ratio increasing. The nuclear diameter decreases and chromatin condenses with the staining reaction progressing from purplish red to dark blue at the final nuclear stage of Orthochromatic erythroblast, prior to nuclear ejection. The colour of the cytoplasm changes from blue at proerthroblast and basophillic stages to a pinkish red as a result of the increasing expression of haemoglobin as the cell develops. Initially, the nucleus is large in size and contains open chromatin. But, as red blood cells mature, the size of the nucleus decreases, until it finally disappears with the condensation of the chromatin material

Why does the nucleus become more heterochromatic during later stages of erythroid differentiation?

The hemoglobin congregates at the periphery of the cell, creating a pinker pigment here and a lighter color in the center.

Why is there such a high proportion of cells of the myeloid lineage in marrow when the proportion in circulation is relatively small compared to erythrocytes?

Cells of the myeloid lineage have shorter lifespans so we need to produce more, while the erythrocytes have an average lifespan of 120 days so we do not need to produce them as often

**In Bone Marrow:** Myeloid precursor cells are typically found in close approximation to the \_\_\_\_\_. As these cells mature, they migrate toward the \_\_\_\_.

bone trabeculae sinusoids

**In the Bone Marrow:** Erythroid precursor cells typically develop away from the ___ , nearer to ___ , in clumps of cells that are dividing and maturing. These are known as \_\_\_.

trabeculae sinusoids erythroid islands

**In the Bone Marrow:** Megakaryocytes are located adjacent to \_\_\_, discharging platelets into circulation.

sinusoids