Histology of Infections in Tissues Flashcards
List three microorganisms which may be detected in tissues.
Viruse
Fungi
Protozoa and parasites
On a H&E-stained slide, the first indication of an infection is the presence of _____________ in the tissue.
Inflammation
List three macroscopic features which may indicate tissue infection.
Frank pus, abscess formation, focal necrosis, and granulomas
True or false: infectious agents may be poorly visualised by H&E, and may require special techniques to demonstrate their presence.
True
Name two stains used to identify H. pylori.
Warthin Starry stain and Giemsa stain
___ ___________ is positively identified as a causative agent of peptic ulcers, and less commonly of gastric cancer.
H. pylori
Histological demonstration of H. pylori depends on demonstration of curved _________ in the biopsy specimen, primarily in the mucus adjacent to epithelial cells.
Bacilli
___ _____________ is the most clinically-significant acid-fast bacillus, and the second most significant infectious cause of death.
M. tuberculosis
What do the cell walls of mycobacteria and related pathogens contain?
Mycolic acids
______ substances allow Mycobacteria to retain stains like carbol fuchsin, even when they are decolourised with strong acid.
Lipid
Most fungal infections affect the __________ skin layers.
Superficial
Fungi are better defined using the _________ and Periodic Acid Schiff (PAS) stains, due to their carbohydrate-rich cell walls.
Grocott
True or false: viruses do not require electron microscopy for detection.
False
________ stain can be useful for staining in viral infections.
Orcein
__-_____ ___________ uses reporter synthetic DNA probes, which hybridise and label specific RNA sequences, in target microbes present in the sample.
In-situ hybridisation
