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CATT > Histology Stains > Flashcards

Histology Stains Flashcards

1
Q

basic dye carries what charge

A

positive

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2
Q

basic dye binds what charge

A

negative

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3
Q

basophilic molecules bind to

A

basic dyes

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4
Q

three examples of basic dyes

A

methylene blue
toludine blue
hematoxylin

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5
Q

nucleic acids

A

basophilic

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6
Q

nuclei

A

basophilic

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7
Q

nucleoli

A

basophilic

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8
Q

ribosomes

A

basophilic

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9
Q

glycoaminoglycans

A

basophilic

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10
Q

acidic dyes carry what charge

A

negative

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11
Q

acidic dyes bind to what charge

A

positive

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12
Q

molecules that acidic dyes bind to are

A

eosinophilic or acidophilic

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13
Q

5 examples of acidic dyes

A 
eosin 
aniline blue
orange G
picric acid 
acid fuchsin
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14
Q

collagen

A

eosinophilic

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15
Q

keratin

A

eosinophilic

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16
Q

hemoglobin

A

eosinophilic

17
Q

muscle proteins

A

eosinophilic

18
Q

describe metachromasia

A

shift in normal color that a dye would be (from blue to purple) in presence of polyanionic substances - super high charge - these molecules polymerize the dye molecules

19
Q

examples of toluidine blue metachromasia

A

mast cell granules (heparin and sulfated proteoglycans)

cartilage extracellular matrix (chondroitin sulfate proteoglycans)

20
Q

what is a Romanovsky type stain

A

a mix of acidic and basic dyes

21
Q

examples of Romanovsky stains

A

Wright Stain, Giemsa, Diffquick

22
Q

what are romanovsky stains typically used for

A

blood smears and cytology

23
Q

what is Periodic Acid Shift

A

used to identify CARBS
adjacent glycol linkages
shows: mucus, glycogen, glycocalyx, polysaccarides in basement membranes, fungal cell walls

24
Q

what is Silver Impregnation

A

looks for reticular fibers and nerve fibers
target stains black
fungi and spirochetes, type III collagen
neurofibrils, basement membrane, golgi

25
Q

what is Elastic Fiber (types and what is does)

A

aldehyde fuchsin, Verhoeff- Van Gieson, Orcein, Weigerts elastin
elastic fibers- stain black

26
Q

what is Trichrome Stain

A

connective tissue
smooth muscle form extracellular collagen
collagen from nerve components
(which are usually hard to distinguish with an H&E stain)
collagen will appear blue green and cytoplasm red/orange to red/lilac

27
Q

Acid Fast Stain

A

organisms with host cell reactions

background will be green with magenta bacteria

28
Q

a mitochondria with H&E will appear

A

grainy eosinophilic

29
Q

an rER with Romanovsky

A

blue tinge

30
Q

Golgi with H&E or Romanovsky

A

appears as a pale empty zone

31
Q

histones with H&E

A

blue

32
Q

melanin with H&E, with Wright/Romanovsky

A

brown / dark-green-gray

33
Q

hemosiderin with H&E , with Prussian Blue

A

yellow/brown, blue/green

34
Q

Prussian Blue stains for

A

Fe, turns blue

CATT (1 decks)

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