Histotechnology Flashcards

Question

What is the primary use of an Ultra-thin Microtome?

Answer 1

Used to produce sections for electron microscopy.

Answer 2

Tissues are embedded in "plastic resins."

Answer 3

Glass knives and Diamond knives.

Answer 4

0.5-1 micron.

Answer 5

500-1200 angstrom (50-120nm).

Answer 6

Length: 25mm Use: For base sledge, rotary and rocking microtome Less concave side: For Celloidin embedded tissue blocks More concave side: For Paraffin embedded tissue blocks

Answer 7

Length: 120mm Use: Rotary microtome Both sides are concave for cutting paraffin embedded sections

Answer 8

Length: 100mm Use: Base sledge or Sliding microtome Both sides are straight For frozen sections or very hard and tough paraffin embedded tissues

Answer 9

Honing (Hard Sharpening) Stropping (Polishing)

Answer 10

Removal of gross nicks Done in heel to toe direction Types of hones used: a. Belgium yellow: Manual sharpening; gives best results b. Arkansas: Gives more polishing effect c. Fine carborundum: Much coarser than the first two; used only for badly nicked knives

Answer 11

Removing of burr (formed during honing) Done to polish and sharpen the cutting edge Done in toe to heel direction

Answer 12

More commonly used Honing and stropping process is eliminated Cheaper to use than conventional steel knives

Answer 13

Can attach to some blade holders Particularly suitable for use in the cryostat

Answer 14

0.5-1 micron thickness Used for trimming and semi-thin "survey" sections of tissue blocks for electron microscopy

Answer 15

500 to 1200 angstrom thickness Used only for cutting "ultra-thin" sections for Electron microscope Used in any type of resin block for EM Brittle; expensive; very durable

Answer 16

27° to 32°

Answer 17

Clearance angle: 0-15° To prevent uneven sections or alternate thin and thick sections, the knife should be inclined with 5-10° clearance angle

Answer 18

Fixation Dehydration (most critical stage) Clearing Infiltration

Answer 19

Constant agitation.

Answer 20

Done under negative atmospheric pressure inside an embedding oven.

Answer 21

Gives fastest result.

Answer 22

Manual embedding.

Answer 23

Used to float out and flatten paraffin ribbons.

Answer 24

Set at a temperature of 45°-50°C.

Answer 25

Approximately 6-10°C lower than melting point of the wax used.

Answer 26

Small amount of detergent may be added to the water to reduce surface tension, allowing the sections to flatten out with greater ease.

Answer 27

Regular at 55-60°C.

Answer 28

Done at 56-60°C for 2 hours.

Answer 29

May be used instead of drying oven Not recommended since they can cause overheating Risk of dust falling onto the section during drying period

Answer 30

Used in handling sections during cutting, and for removing folds and creases on the sections during "floating out" in water bath.

Answer 31

76x25mm slides that are 1.0 to 1.2 thick are preferred Frosted end are preferred, where the ID number of the section can be inscribed with a pencil Slide refractive index: 1.518

Answer 32

Coplin jars: Slotted jar holding 5 to 9 slides Slotted staining dishes: Holding 5 to 19 slides, over which different solutions are poured Metal or Glass staining racks or carriers: Holding 10 to 30 slides upright

Answer 33

Fine Needle Aspiration Incisional Biopsy Excisional Biopsy Punch Biopsy Shave Biopsy Curetting

Answer 34

Simplest, least invasive test that uses the smallest needle to remove cells from the area of abnormality.

Answer 35

Removes not only the cells, but also the small amount of surrounding tissues Provides additional information to assist in the examination of the lesion

Answer 36

Takes out even more surrounding tissue Takes out some of the abnormality, but not all The doctor will slice into the lesion and remove only the lesion or only a portion of it

Answer 37

Generally removes the entire area in question.

Answer 38

A diagnostic test where a small, tube-shaped piece of skin and some other tissue underneath are removed using a sharp cutting tool Can be done anywhere on the body 3 to 4mm cylindrical core of tissue must be obtained

Answer 39

A diagnostic test where a thin piece of skin is removed from the surface using a sharp blade.

Answer 40

A loop, ring, or spoonshaped instrument, attached to a handle and having sharp or blunt edges; used to scrape tissue from a surface.

Answer 41

Selected tissue specimen is immersed in a watch glass slide containing isotonic salt solution (NSS), carefully dissected or separated and examined under the microscope.

Answer 42

The smearing or compressing of a thin tissue specimen between two slides for microscopic analysis. The specimen is placed flat on the first slide. The second slide is held at a right angle to the first and then dragged along the specimen, distributing it lengthwise along the first slide.

Answer 43

Useful in cytological examination particularly for cancer diagnosis.

Answer 44

Used of an applicator stick or platinum loop, material is rapidly and gently applied in a direct or zigzag line throughout the slide.

Answer 45

Material is transferred to a clean slide and gently spread into a moderately thick film by teasing using an applicator stick Recommended for fresh sputum, bronchial aspirates and thick mucoid secretions

Answer 46

A drop of secretion or sediment upon 1 slide and facing it to another clean slide 2 slides are pull apart with a single uninterrupted motion

Answer 47

Special method of smear preparation whereby the surface of the freshly cut tissue is brought into contact and pressed on the surface of a clean glass slide.

Histotechnology Flashcards

(72 cards)