HMO: FS G-B Flashcards
Haemophilus, Brucella, Bordetella, Francisella, Pasteurella, Legionella, Capnocytophaga (37 cards)
Fastidious organism can grow on:
prefers incubation at 35-37degC except:
Unable to grow on
Horse and Rabbit’s Blood
H. ducreyi highly FASTIDIOUS, 33degC
SBA
heat stable released upon degredation of hemoglobin
HEMIN ‘‘X FACTOR’’
heat labile
provided by SAU, S. pneumoniae, Neisseria, Yeast, Potato extract
NAD ‘‘V FACTOR’
CO-ENZYME I
Gram negative, NM bacilli and coccobacilli, Pleomorphic
Haemophilus
most important spp. causing meningitis, otitis media, epiglottis, pneumonia, contagious conjunctivitis
H. influenzae
SOFT CHANCRE
SCHOOL OF FISH/ FINGERPRINT/ RAILROAD TRACK
H. ducreyi
’‘KOCH-WEEKS BACILLUS’’
PINK-EYE
BRAZILIAN PURPURIC FEVER affects children
H. aegypticus
RED EYE: Adenovirus
Haemophilus spp.
normal flora of URT
Found in dental plaque
H. parainfluenzae
H. aphrophilus
seen primarily in ENDOCARDITIS
most common cause of disseminated infections but widespread immunization with the Hib vaccine
H. influenzae Serotype B
GROWTH can also be seen surrounding colonies of SAU, S. pneumoniae, Neisseria spp
SATELLITISM
sensitive method to determine X FACTOR requirements
Porphyrin test
requires both X and V FACTOR:
Catalase +:
Glucose -:
Porphyrin +:
H. influenzae, H. aegypticus, H. haemolyticus
H. influenzae, H, aegypticus
H. ducreyi
H. parainfluenzae and H. aphrophilus
normal flora in the respiratory tract of animals, Infected DOGS and CAT bite or scratch
Pasteurella multocida
small gram negative coccobacillus with BIPOLAR STAINING
BAP: musty or mousey smell
P. multocida
stains poorly with conventional GS, intracellular
NM, strict Aerobes
Bioterrorism agent except:
Category:
Brucella
Brucella canis
B
Growth of Brucella is inhibited by:
Basic fuchsin & Thionine
Brucella
in animals causes:
in Man it causes:
Abortion
BRUCELLOSIS UNDULANT FEVER/, MALTA FEVER, GIBRALTA FEVER, MEDITERRANEAN FEVER
MOT:
1. INHALATION
2. DIRECT CONTACT w/ animals
3. INGESTION of contam meat/dairy product
Optimal specimen for diagnosis of Brucella:
Rapid test to detect Brucellosis:
- Significant titer:
- Species not detected by this method
- Media for Isolation:
BLOOD
SERUM AGGLUTINATION TEST
1:160
B. canis
CANTANEDA BOTTLES 3-4 hrs
BANG’S BACILLUS Cows, Cattle:
Sheep, Goats:
Pig, Swine:
Dogs:
Brucella abortus
B. mellitensis
B. suis
B. canis
*small * G-b Strict Aerobe replicates ciliated respiratory ET cells
Bordetella
Growth of Bordetella can be accomplished using:
REGAN-LOWE or BORDET-GENGOU agar
charcoal Horse blood
stage of pertusis most infectious
spread through:
BEST SPECIMEN:
CATARRHAL
Droplet
NASOPHARYNGEAL SWAB
MERCURY DROP colonies
PEARL-LIKE in appearance in 3-4 days
NG in BAP
Urease -
B. pertussis
causes respiratory tract disease in Animals
KENNEL COUGH
B. bronchiseptica