HPLC Flashcards

1
Q

Schema a blocchi hplc

A
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2
Q

Difficolta’ hplc

A
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3
Q

Tipi di ionizzazione a pressione atmosferica hplc

A
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4
Q

ESI, i tre tipi e i flussi rispettivi hplc (cono di taylor), struttura

A
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5
Q

Miscelatore a bassa ed a altra pressione hplc

A
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6
Q

Solventi da usare, e caratteristiche della fase mobile hplc

A
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7
Q

Modalita’ di lavoro isocratica e a gradientre

A
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8
Q

Serie eluotropica

A
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9
Q

Tipi di pompe e vantaggi hplc

A
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10
Q

Iniettori hplc

A
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11
Q

colonne hplc con rispettivi flussi

A
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12
Q

Particelle impaccanti hplc e numero di piuatti teorici

A
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13
Q

Isoterma di vna deemter, e compromesso accettabile con HPLC e p[articelle impaccanti piccole

A
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14
Q

Materiale impaccante: silice nuda o funzionalizzata, range di ph

A
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15
Q

LSC, adsorbenti usati (carbone, silice ecc…), tailing e fronting (moticvazioni)

A
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16
Q

Impaccamento polimerico o silice, versalitita’ del c18, vantaggi

A
17
Q

cromatografia a scambio ionico (controione, analita, eluente (forza ioinca maggiore o forza di massa)

A
18
Q

Struttura APCI, t alte, Vantaggi (polarita’ di molecole trattabili da apci rispetto a ESI)

A