human insulin from bacetria

Question 1

summarise the making of human insulin from bacteria

Answer 1

sticky ends from human insulin gene and bacteria plasmid is cut via restriction enzymes, to repair the sugar phosphate backbone via DNA ligase, to form a recombinant plasmid. bacteria cell is induced to take up the plasmid and undergoes screening where only cells that have antibiotic resistance gene is the plasmid survive and grow. the bacteria cells are cultured in a fermenter indistrially, and DNA inside the cells undergo transcription and translation to form proteins, which are isolated and crystalised