IgM and IgG

Question 1

Classify the RBC groups as IgG or IgM; ABO, Rh, lewis, Ii, P, MNSs, Kell, Duffy, Kidd.

Answer 1

M, G, M, M, M, M, G, G, G

Question 2

What are the 7 Common characteristics to IgMs?

Answer 2

Large Molecule, Binds Complement effectively, Direct agglutination at IS and room temp/colder is optimal, Naturally occurring, do not cross placentas and other than ABO are not clinically significant

Question 3

What is different about Lewis antibodies?

Answer 3

They are mad by tissues and released into blood to be incorporated into the RBC membrane; like Velcro

Question 4

What enzyme adds to what precursor to make Le?

Answer 4

PS—-(Fucosyl trasnferase)—> Le^a antigen

Question 5

If you have Le, Se and H what is your lewis Gene?

Answer 5

Le(a-b+)

Question 6

If you have Le, sese and H what is your lewis gene?

Answer 6

Le(a+b-)

Question 7

If you have Le Se and hh what is your lewis gene?

Answer 7

Le(a+b-)

Question 8

If you have lele, Se or se / h or H what is your lewis gene?

Answer 8

Le(a-b-)

Question 9

Look at slide 14 and fill in

Answer 9

Review

Question 10

What are two reasons for lewis not being involved in HDN?

Answer 10

IgM (do not cross placenta) and they are not developed at birth so there is nothing to attach to

Question 11

What precursor is I acting on?

Answer 11

PS1

Question 12

What is the expression of I and i on adult cells?

Answer 12

I is on adult

Question 13

What is the expression of I and i on cord cells?

Answer 13

i is on cord cells

Question 14

At what age is Ii heterozygous seen?

Answer 14

2

Question 15

What are the main pathological diseases with Anti-i and anti-I?

Answer 15

Little i: Infectious monoculeosis

I: Micoplastic Pneumoniae (Cold hemagglutinin Disease)

Question 16

What phenotype is P1 like?

Answer 16

A1-> P1, P and Pk antigens

Question 17

What phenotype is P2 like?

Answer 17

A2-> P2, P and Pk antigens

Question 18

Where are P ags found?

Answer 18

on RBC

Question 19

When are P ags developed and when are they fully expressed?

Answer 19

Fully developed AFTER birth and will have full expression at age 7

Question 20

What is used to neutralize anti P1 or anti-P2 in antibody identifications?

Answer 20

P substance from Pigeon droppings

Question 21

What phenotype can make anti P1?

Answer 21

P2

Question 22

What disease is associated with P antibody?

Answer 22

PCH

Question 23

Where are M & N antigens found and when are they fully developed?

Answer 23

Glycophorin A; developed at Birth

Question 24

How are M and N inherited, what are they referred to as?

Answer 24

Mary and Nacy are inherited as a packet, similar to Rh

Question 25

What are the 7 characteristics of IgGs?

Answer 25

Red Cell Stimulated, small in size (no direct agglutination) can cross the placenta, Warm-37, Do NOT bind complement, need AHG to see, cause HTR

Question 26

What is the most common phenotype in Kell?

Answer 26

K-K+, Kp(a-b+), Js(a-b+)

Question 27

What are the ag frequencies with Kell?

Answer 27

K=9%, k=98.8%, Kpa 2%W, Kpa>99.9%, Jsa99.9W or 99% B

Question 28

THIRD OR FIRST immunogenic ??? wha.....

Answer 28

IMMUNOGENEIC, she only gives us third

Question 29

What is the McLeod Phenotype ag expression and what disease is this associate with?

Answer 29

weakened expression of all K ags, Chronic Granulomatous Disease

Question 30

Compare Fy(a+b-) or Fy(a-b+) in Caucasians with Fy(a+b-) or Fy(a-b+) in AA with regard to genes producing the phenotype.

Answer 30

Look at slide 19 in PP

Question 31

What is the correlation of Fy(a-b-) in AA?

Answer 31

Linked to resistance of malaria (plasmodium vivax)

Question 32

What are Antibodies of Kidd "bad?" Why?

Answer 32

They are notorious for causing severe HTR, play hide and seek, usually seen with other abs and don't like to play alone, only super responders make them, enhanced with enzyme treatment, bind complement

Question 33

What antibodies commonly bind complement?

Answer 33

Kidd and Lewis

Question 34

Correlation of U ag with S and s.

Answer 34

U is not found on S and s but is found on all other antigens of MNs

Question 35

What are the three sources from which compatible RBCs can be obtained with clinically sig. antibody to high frequency ag is found?

Answer 35

Autologous, siblings and rare donor file

Question 36

What is the blood group system associated with the HLA system?

Answer 36

Bg Ags

Question 37

What blood group system is sex linked?

Answer 37

Anti Xga

Question 38

Name three systems besides the ABO in which soluble antigens occur.

Answer 38

Lewis, Ii, P

Question 39

Name three blood systems with significant racial variation.

Answer 39

Lewis, Kell, Duffy

Question 40

Five antigens destroyed by enzymes

Answer 40

Fya, Fyb, M, N, S

Question 41

What is HLA?

Answer 41

High leukocyte antigen

Question 42

What is HTLA?

Answer 42

High Titer low avidity