IHC Flashcards
1
Q
What markers are associated with Squamous cell carcinoma?
A
high molecular weight cytokeratins, EMA
2
Q
What markers are used to identify Melanoma?
A
S100, MELAN-A, HMB-45
3
Q
Identify the markers for Mesothelioma.
A
calretinin, CK5/6
4
Q
What are the markers for Adenocarcinoma lung?
A
CK7+, CK20-, TTF+
5
Q
What markers characterize Adenocarcinoma colon?
A
CK7-, CK20+, TTF1-, CDX2+
6
Q
Which markers are associated with Neuroendocrine tumors?
A
chromogranin, synaptophysin, neuron specific enolase
7
Q
What markers are used to identify GIST?
A
CD117, PDGFRa
8
Q
List the markers for Hepatocellular carcinoma.
A
HePAR1, AFP, EMA, pankerattin
9
Q
What markers are associated with Adrenocortical neoplasms?
A
inhibin, melanA
10
Q
Identify the markers for Pheochromocytoma.
A
NSE, chromogranin, synaptophysin, opiod peptides
11
Q
What markers characterize Renal cell carcinoma?
A
CK8, CK18, CD10, RCC antigen
12
Q
List the markers for Transitional cell carcinoma.
A
thrombomodulin, uroplakin
13
Q
What markers are associated with Seminoma?
A
PLAP, CD117
14
Q
What markers are used to identify Embryonal carcinoma?
A
CD30
15
Q
What is the marker for Yolk sac tumor?
A
AFP
16
Q
Identify the markers for sex cord stromal tumors.
A
Inhibin, calretinin, activin, mullerian inhibiting substance
17
Q
What markers are associated with Leiomyoma?
A
smooth muscle actin, caldesmon, calponin, desmin
18
Q
What markers characterize Serous ovarian tumors?
A
CK7+, CK20-
19
Q
What markers are used for Invasive ductal carcinoma of the breast?
A
ER, PR, Her2neu
20
Q
What is a specific marker for Invasive lobular carcinoma?
A
absence of Ecadherin
21
Q
What does CD45 represent?
A
common leucocyte antigen
22
Q
List the Pan B markers.
A
CD19, CD20, CD21, CD22, PAX5, CD79a
23
Q
List the Pan T markers.
A
cytoplasmic CD3, CD2, CD5
24
Q
What markers are associated with NK cells?
A
CD56, CD16
25
What markers are used for Plasma cells?
CD138, immunoglobulin kappa/lambda light chains, MUM1
26
What is the marker for ALL and Burkitt's lymphoma?
CD10 positive
27
What does Tdt positivity indicate?
ALL
28
What markers are associated with Hodgkin's lymphoma?
CD15, CD30 - CLASSIC
29
What markers characterize Anaplastic large cell lymphoma?
CD30+, ALK1, EMA+
30
What markers are used for Mantle cell lymphoma?
cyclinD1, CD5+, pan B markers
31
What markers are associated with Langerhans cell histiocytosis?
CD1a, S100, LANGERIN
32
What markers are used for Hairy cell leukemia?
CD19, CD20, CD22, CD11c, CD25, CD103
33
What markers are associated with Ewing's sarcoma?
CD99, vimentin, NSE
34
What does Cytokeratin indicate?
Carcinomas, epithelioid sarcoma, synovial sarcoma, some angiosarcomas, leiomyosarcomas, mesothelioma, extrarenal rhabdoid tumor
35
What does Vimentin indicate?
Sarcomas, melanoma, some carcinomas and lymphomas
36
What does Desmin indicate?
Benign and malignant smooth and skeletal muscle tumors
37
What does Glial fibrillary acidic protein indicate?
Gliomas, some schwannomas
38
What do Neurofilaments indicate?
Neuroblastic tumors
39
What does Pan-muscle actin indicate?
Benign and malignant smooth and skeletal muscle tumors, myofibroblastic tumors and pseudotumors
40
What does Smooth muscle actin indicate?
Benign and malignant smooth muscle tumors, myofibroblastic tumors and pseudotumors
41
What do Myogenic nuclear regulatory proteins indicate?
Rhabdomyosarcoma
42
What does S-100 protein indicate?
Melanoma, benign and malignant peripheral nerve sheath tumors, cartilaginous tumors, normal adipose tissue, Langerhans cells
43
What does Epithelial membrane antigen indicate?
Carcinomas, epithelioid sarcoma, synovial sarcoma, perineurioma, meningioma, anaplastic large cell lymphoma
44
What does CD31 indicate?
Benign and malignant vascular tumors
45
What does von Willebrand factor indicate?
Benign and malignant vascular tumors
46
What does CD34 indicate?
Benign and malignant vascular tumors, solitary fibrous tumor, hemangiopericytoma, epithelioid sarcoma, dermatofibrosarcoma protuberans
47
What does CD99 indicate?
Ewing's sarcoma/primitive neuroectodermal tumor, some rhabdomyosarcomas, some synovial sarcomas, lymphoblastic lymphoma, mesenchymal chondrosarcoma, small cell osteosarcoma
48
What does CD45 indicate?
Non-Hodgkin lymphoma
49
What does CD30 indicate?
Anaplastic large cell lymphoma, embryonal carcinoma
50
What does CD68 indicate?
Macrophages, fibrohistiocytic tumors, granular cell tumors, various sarcomas, melanomas, carcinomas
51
What are Melanosome-specific antigens used for?
Melanoma, PEComa, clear cell sarcoma, melanotic schwannoma
52
What do MDM2/CDK4 indicate?
Atypical lipomatous tumor and dedifferentiated liposarcoma
53
What does Glut-1 indicate?
Perineurioma, infantile hemangioma
54
What does Protein kinase C ϑ indicate?
GIST
55
What does Bcl-2 indicate?
Synovial sarcoma, solitary fibrous tumor, other spindle cell tumors
56
What is the normal cell counterpart for Angiosarcoma?
Endothelium
57
What is the useful marker for Angiosarcoma?
CD31, CD34, FLI-1, von Willebrand factor, ulex lectin
58
What is the normal cell counterpart for Leiomyosarcoma?
Smooth muscle
59
What are the useful markers for Leiomyosarcoma?
Muscle (smooth) actins, desmin, caldesmon, myosin heavy chain
60
What is the normal cell counterpart for Rhabdomyosarcoma?
Skeletal muscle
61
What are the useful markers for Rhabdomyosarcoma?
MyoD1, myogenin; muscle (sarcomeric) actins; desmin
62
What is the normal cell counterpart for Malignant peripheral nerve sheath tumor?
Nerve sheath (e.g., Schwann cell, perineurial cell)
63
What are the useful markers for Malignant peripheral nerve sheath tumor?
S-100, CD57, NGF receptor, EMA, claudin-1, Glut-1
64
What is the normal cell counterpart for Liposarcoma?
Adipocyte
65
What are the useful markers for Liposarcoma?
S-100 protein, MDM2, CDK4
66
What is the normal cell counterpart for Chondrosarcoma?
Chondrocyte
67
What are the useful markers for Chondrosarcoma?
S-100 protein
68
What is the normal cell counterpart for Osteogenic sarcoma?
Osteocyte
69
What is the useful marker for Osteogenic sarcoma?
Osteocalcin
70
What is the normal cell counterpart for Kaposi sarcoma?
Endothelium
71
What are the useful markers for Kaposi sarcoma?
CD31, CD34, VEGFR3, LANA
72
What is the normal cell counterpart for Myofibroblastic lesions?
Myofibroblast
73
What are the useful markers for Myofibroblastic lesions?
Smooth muscle actins
74
What is the normal cell counterpart for Gastrointestinal stromal tumor?
Interstitial cells of Cajal
75
What are the useful markers for Gastrointestinal stromal tumor?
CD117a (c-kit), CD34, protein kinase C ϑ
76
What is the normal cell counterpart for Hemangiopericytoma?
?
77
What are the useful markers for Hemangiopericytoma?
CD34, bcl-2
78
What is the normal cell counterpart for Glomus tumors?
Glomus cell
79
What are the useful markers for Glomus tumors?
Smooth muscle actins, type IV collagen
80
What type of inclusions does pox virus cause?
Cytoplasmic
81
What type of inclusions does herpes virus cause?
Nuclear
82
What type of inclusions does measles cause?
Both nuclear and cytoplasmic
83
What type of inclusions are associated with adenovirus?
Basophilic inclusions
84
What are Negri bodies?
Intracytoplasmic eosinophilic inclusions in rabies
85
What are guaneri bodies?
Intracytoplasmic multiple smaller inclusions in vaccinia
86
What are Bollinger bodies?
Fowl pox
87
What are Molluscum bodies?
Larger intracytoplasmic inclusions in molluscum contagiosum
88
What are Cowdry type A inclusions associated with?
Herpes virus, yellow fever virus
89
What are Cowdry type B inclusions associated with?
Adenovirus, polio virus
90
What type of inclusions does Cytomegalovirus cause?
Both basophilic intranuclear inclusion and eosinophilic cytoplasmic inclusion
91
What inclusions are associated with Parvo virus B19?
Intranuclear inclusions in erythroblasts
92
What are Donovan bodies?
Calymmatobacterium granulomatis
93
What inclusions does Chlamydia trachomatis cause?
Halbersteadter Prowazek bodies
94
What are Miyagawa’s granulocorpuscles?
Chlamydia trachomatis causing lymphogranuloma venereum
95
What are Levinthal cole lilies bodies?
Chlamydia psittaci causing psittacosis
96
What are Sclerotic / copper penny/ meddlers bodies associated with?
Chromoblastomycosis
97
What are Maltese crosses seen in?
Peripheral smear - RBCs - babesiosis
98
What are Civatte (colloid) bodies associated with?
Lichen planus
99
What are Kamino bodies associated with?
Spitz nevus
100
What inclusions are seen in Alzheimer's disease?
Senile neuritic plaques & neurofibrillary tangles
101
What are Hirano bodies associated with?
Alzheimer's disease - elongated eosinophilic glassy inclusions
102
What are Pick bodies associated with?
Pick disease - cytoplasmic filamentous inclusions in the neurons
103
What are Aschoff bodies associated with?
Rheumatic fever
104
What are Russel bodies?
Intracytoplasmic inclusions in plasma cell myeloma
105
What are Dutchers bodies?
Intranuclear inclusions in plasma cell myeloma
106
What are Gamna gandy bodies associated with?
CVC spleen
107
What are Mallory bodies associated with?
Alcoholic liver disease
108
What kind of lesions are Onion skin lesions?
Hypertensive arteriolosclerosis
109
What does Stag horn appearance indicate?
Hemangiopericytoma
110
What does Bovine heart appearance indicate?
Syphilis
111
What does Tree bark appearance of vessel indicate?
Syphilitic aneurysm
112
What does Banana shaped ventricular cavity indicate?
Hypertrophic cardiomyopathy
113
What are Skip lesions associated with?
Crohn's disease
114
What does Creeping fat indicate?
Crohn's disease
115
What does Cobblestone appearance indicate?
Crohn's disease
116
What are Pseudopolyps associated with?
Ulcerative colitis
117
What does Arborizing pattern indicate?
Peutz Jeghers polyp
118
What does Row of tombstone appearance indicate?
Pemphigus vulgaris
119
What does Dilapidated brick wall appearance indicate?
Hailey Hailey disease
120
What are Corps ronds and grains associated with?
Dariers disease
121
What does Palisaded pattern indicate?
Schwannoma
122
What does Herring bone pattern indicate?
Fibrosarcoma
123
What does Storiform pattern indicate?
Fibrohistiocytoma
124
What are Rosettes associated with?
Flexner winterstriner rosettes - retinoblastoma
125
What are Homer Wright rosettes associated with?
Neuroblastoma
126
What are Fleurettes associated with?
Retinoblastoma
127
What does Crookes hyaline change indicate?
ACTH adenoma
128
What does Tennis racket appearance indicate?
Birbeck bodies - Langerhans cell histiocytosis
129
What does Wind blown appearance of nuclei indicate?
Bowen's disease
130
What does Zellballen pattern indicate?
Paraganglioma
131
What does Swiss cheese appearance indicate?
Cystoglandular hyperplasia of endometrium
132
What does Shredded carrot appearance indicate?
Neurofibroma
133
What does Plexiform vasculature /crow feet vessels indicate?
Myxoid liposarcoma
134
What does Chicken wire calcification indicate?
Chondroblastoma
135
What does Gram stain identify?
Gram positive bacteriae, actinomyces
136
What does Acid fast stain identify?
Mycobacteria, nocardiae
137
What does Silver stain identify?
Fungi, legionellae, pneumocystis
138
What does Periodic acid stain identify?
Fungi, amoebae, to delineate basement membrane, glycogen
139
What does Mucicarmine stain identify?
Cryptococcus
140
What does Giemsa stain identify?
Helicobacter pylori, leishmania, malarial parasites
141
What does Perls stain identify?
Iron stain - ringed sideroblasts in myelodysplastic syndrome, increased in hemochromatosis, seen in hemosiderin laden macrophages
142
What does Masson Fontana stain identify?
Stains for melanin
143
What does PAS positive indicate?
Glycogen storage diseases - Gaucher disease
144
What does Sudan black B stain identify?
Lipid storage diseases - Niemann Pick disease
145
What does Oil red O stain identify?
Neutral fat in Burkitt's lymphoma
146
What does Myeloperoxidase staining indicate?
In AML
147
What does Van Geison's stain identify?
Elastin (black colour)
148
What does Mason's trichrome stain identify?
Collagen (green/blue), muscle (red), nuclei (yellow)
149
What does Congo red stain identify?
Amyloidosis - apple green birefringence
150
What does Reticulin stain indicate?
In myelofibrosis for grading
151
What does Indian ink staining identify?
Cryptococcus
152
What does Rubeanic acid staining identify?
Copper in Wilson's disease
153
What does TRAP Positive indicate?
Hairy cell leukemia
154
What does Von Kossa and Alizarin red S stain identify?
Calcium stain
