Immunophenotypes of various diseases Flashcards
(12 cards)
1
Q
Acute lymphoblastic leukemia
- B cells
- surface light chain?
- T cells
- both
A
- B: CD22, 19, 10, 79,
- surface light chain negative
- T: markers 2-8, NOT 10
- TdT and D34
2
Q
Chronic lymphoblastic leukemia
- B or T cells:
- immunophenotype
- light chain status
A
- naive B cells
- express CD19, 5***, 23, 20
- CD5 is a T cell marker
- dim light chain due to naive B cells
3
Q
Follicular lymphoma
- T or B cells
- location in lymph node
- immunophenotype
- light chain status
- which proteins do these cells overexpress? (think anti-apoptosis)
- what distinguishes FL from ALL? CLL?
A
- B cells
- located in the germinal center, hence the name
- CD19, 20, 10 (similar to ALL, but missing CD 79)
- light chain BRIGHT (they’ve been exposed to antigen)
- bcl-2 and bcl-6
- distinction from ALL: light chain bright
- distinction from CLL: CD5 negative
4
Q
Mantle cell lymphoma
- immunophenotype
- light chain status
- overexpressed protein product
A
- CD19, CD20, CD5
- light chain BRIGHT
- cyclin D
5
Q
hairy cell leukemia
- immunophenotype
- rare mutation
A
- CD19, 20, 11c, 25, 103
- BRAF mutation
6
Q
plasma cell neoplasms
- immunophenotype
- light chain status
A
- CD38, 56
- CD19, 20, and 45 negative!
- intracellular light chain
7
Q
diffuse large B cell lymphoma
- immunophenotype
- light chain
- where do you find it?
- cytogenetics
A
-CD19, 20
+/- bcl2, bcl6, CD10
-light chain restricted
-all over the place in a rapidly enlarging symptomatic mass
8
Q
Burkitt lymphoma
- immunophenotype
- light chain
- bcl2?
A
- CD19, 20, 10
- lc restriction
- no bcl2
9
Q
anaplastic large cell lymphoma
-which protein serves as the tyrosine kinase?
A
- CD30, EMA
- ALK
10
Q
adult t cell leukemia/lymphoma
-think of the carribean basis and Japan
A
- cd4+/CD7-
- HTLV-1 +
11
Q
hodgkin lymphoma
- famous cells
- B or T cells
- immunophenotype
A
- reed sternberg cells
- B cells with a rearranged Ig
- CD15, 30
12
Q
NLPHL
A
- B cell antigen +, CD45
- bcl6
- CD15/30-
