Infiltration/Impregnation

Question 1

Purpose of infiltration

Answer 1

To remove clearing agent from tissues
To fill cavities and tissue spaces to give firm consistency for easy cutting

Question 1

Purpose of infiltration

Answer 1

To remove clearing agent from tissues
To fill cavities and tissue spaces to give firm consistency for easy cutting

Question 2

Infiltration Methods:

Answer 2

1. Manual
2. Automatic
3. Vacuum

Question 3

Paraffin wax substitute: (4)

Answer 3

1. Paraplast
2. Bioloid
3. Carbowax
4. Ester Wax

Question 4

Infiltration under negative atmospheric pressure inside the oven

Answer 4

Vacuum

Question 5

Most rapid method as it subject tissues to a very high temperature
that facilitates removal of transition solvents and prolongs life of wax
by preventing solvent contamination

Answer 5

Vacuum

Question 6

Uses autotechnicon which requires 3-4 changes of wax

Answer 6

Automatic

Question 7

Temperature range for manual infiltration

Answer 7

55-60°C

Question 8

Approximate temperature when using manual infiltration

Answer 8

2-5°C higher than the wax melting point

Question 9

Recommended for bones

Answer 9

Paraplast

Question 10

Melting point of Paraplast

Answer 10

56-57°C

Question 11

Substitute for paraffin wax used for eye specimens

Answer 11

Bioloid

Question 12

Paraffin substitute that is water soluble

Answer 12

Carbowax

Question 13

What is carbowax?

Answer 13

A polyethylene glycol that has 18 or more carbon atoms

Question 14

Appears solid at room temperature

Answer 14

Carbowax

Question 15

Recommended for enzyme histochemistry

Answer 15

Carbowax

Question 16

Melting point of Ester Wax

Answer 16

46-48°C

Question 17

Substitute for paraffin wax that requires heavy duty microtome

Answer 17

Ester wax

Question 18

Recommended for specimens with large and hollow cavities that tends to collapse

Answer 18

Celloidin/Collodion infiltration

Question 19

A very slow process which involves submerging of tissue in different concentration

Answer 19

Celloidin infiltration

Question 20

Recommended for whole eye specimens

Answer 20

Dry celloidin

Question 21

Dry celloidin require the use of what mixture to store blocks

Answer 21

Gilson’s mixture

Question 22

Celloidin infiltration method for bones, brain, & teeth specimens

Answer 22

Wet celloidin

Question 23

Wet celloidin require the use of which agent to store blocks?

Answer 23

70-80% alcohol

Question 24

More preferred than usual celloidin as it produces harder blocks and allows cutting of thinner section

Answer 24

Low Viscosity Nitrocellulose (LVN)

Question 25

Soluble in equal concentration of ether and alcohol

Answer 25

Low Viscosity Nitrocellulose (LVN)

Question 26

Has a lower viscosity which causes higher concentration and can still penetrate tissues rapidly

Answer 26

Low Viscosity Nitrocellulose (LVN)

Question 27

Disadvantage of Low Viscosity Nitrocellulose (LVN) and its remedy

Answer 27

Tissues have tendency to crack Remedy: Add plasticizers (Oleum Ricini & Castor oil)

Question 28

Infiltration method for Enzyme and Histochemical studies

Answer 28

Gelatin infiltration

Question 29

This is only done when dehydration is to be avoided

Answer 29

Gelatin infiltration

Question 30

In gelatin infiltration method, this can be used to prevent growth of molds

Answer 30

Phenol

Question 31

Gilson mixture components

Answer 31

Chloroform + Cedar wood oil