Instrument Used in CC1

Question 1

Required for preparation of any primary standard. Single pan enclosed by sliding transparent doors.

Answer 1

Analytical balances

Question 2

Single pan balances. Uses an electromagnetic force to counterbalance the weighed sample’s sample.

Answer 2

Electronic balances

Question 3

Consists of a head or rotor, carriers, or shields that are attached to the vertical shaft of a motor or air compressor and enclosed in a metal covering

Answer 3

Clinical centrifuge

Question 4

process in which centrifugal force is used to separate solid matter from a liquid suspension; also separate two liquid phases of different densities.

Answer 4

Centrifugation

Question 5

RCF in grams

Answer 5

1.118 x 10-5 x r x (rpm)2 ; or use nomogram

Question 6

RPM

Answer 6

tachometer or strobe light

Question 7

tubes placed in the cups of the rotor assume a horizontal plane when the rotor is in motion and vertical position when at rest.

Answer 7

Horizontal-head or swinging-bucket centrifuge

Question 8

During centrifugation, particles travel in a constant manner along the tube while the tube is at right angle to the shaft of the centrifuge. The surface of the sediment is flat.

Answer 8

Horizontal-head or swinging-bucket centrifuge

Question 9

Tubes are held at a fixed angle from 25-40 degrees to the vertical axis of rotation.

Answer 9

Angle-head or fixed angle centrifuge

Question 10

Particles are driven outside and bottom of the tube and the surface of the sediment packs against the side and bottom of the tube and the surface of the sediment is parallel to the shaft of the centrifuge.

Answer 10

Angle-head or fixed angle centrifuge

Question 11

High-speed centrifuge; mainly fixed angle rotors requires a refrigerated chamber.

Answer 11

Ultracentrifuge

Question 12

circulating or noncirculating. maintenance: Type II (or Type I) water

Answer 12

Water bath

Question 13

List of Transfer Pipets

Answer 13

1. Volumetric or Transfer pipet
2. Ostwald-Folin pipet
3. Pasteur pipets
4. Automatic (macro or micropipets)

Question 14

 used to transfer aqueous solutions & non-viscous samples
 self-draining

Answer 14

Volumetric or Transfer pipet

Question 15

 has the greatest degree of accuracy and precision
 read at the lower meniscus
 with bulb at the center

Answer 15

Volumetric or Transfer pipet

Question 16

 used for biologic fluids having viscosity greater than water
 blowout pipets

Answer 16

Ostwald-Folin pipet

Question 17

 read on the upper meniscus
 bulb is closer to the delivery tip

Answer 17

Ostwald-Folin pipet

Question 18

no calibration mark; used to transfer solutions or biologic fluids without consideration of a specific volume

Answer 18

Pasteur pipets

Question 19

capable of dispensing several used to deliver a predetermined volume of liquid.

Answer 19

Graduated or Measuring Pipets

Question 20

 calibrated between two marks; deliver between their calibration marks
 Tip should NOT touch the receiving vessel
 self-draining; with smaller orifice

Answer 20

Mohr pipet

Question 21

 graduated down to the tip
 blow-out pipet; with larger orifice

Answer 21

Serological pipet

Question 22

exact volume is calibrated to fill the volume between two calibration points on the pipet

Answer 22

“Between Two Marks”

Question 23

 Holds the particular volume but does not dispense the exact volume
 Requires rinsing
 calibrated with mercury
 usually a micropipette - Volumes are expressed in microliter

Answer 23

“To-Contain” (TC)

Question 24

 Delivers the exact volume indicated; Calibrated for the volume delivered
 fluid is allowed to flow freely with the pipet tip touching the inner wall of receiving vessel

Answer 24

“To-Deliver” (TD)

Question 25

 Safer, less time consuming, precise & convenient  mechanism draws up and dispenses the liquid

Answer 25

Automatic macropipets or micropipets

Question 26

relies on a piston for suction creation to draw the sample into a disposable tip.

Answer 26

Air-displacement

Question 27

operates by moving the piston in the tip or barrel.  Sample enters directly upon contact without air interference.  NO need to replace delivery tip.

Answer 27

Positive displacement

Question 28

obtain the liquid from a common reservoir and dispense it repeatedly; combines sampling & dispensing functions

Answer 28

Dilutor/Dispenser pipets

Question 29

delivering and weighing a solution of known specific gravity.

Answer 29

Gravimetric Method

Question 30

the molar extinction coefficient of a compound, such as potassium dichromate is obtained.

Answer 30

Spectrophotometric Method -

Question 31

 has a round, lower portion with with a flat bottom and a long, thin neck with an etched calibrated line

Answer 31

Volumetric Flask

Question 32

 For preparation of standards for quantitative procedures.  Class A , TC (To-Contain)

Answer 32

Volumetric Flask

Question 33

cylindrical shaped calibrated plastic or glass containers.

Answer 33

Centrifuge Tube

Question 34

 13mm x 100mm  Constant light path: 1 cm distance

Answer 34

Cuvets

Question 35

used to contain acidic solutions

Answer 35

SOFT

Question 36

“Borosilicate” for alkaline solutions

Answer 36

HARD

Question 37

TYPES as to COMPOSITION:

Answer 37

Soft, Hard

Question 38

TYPES as to WAVELENGTH SETTINGS

Answer 38

Glass, Quartz

Question 39

TYPES as to SHAPE

Answer 39

Round, Square and Rectangle

Question 40

1. GLASS: for ____________ settings (____ – 700 nm or ____ – 700 nm) 2. QUARTZ: for ___________________ settings (UVR: below ___ nm)

Answer 40

1. GLASS: for wide-range settings (300 – 700 nm or 380 – 700 nm) 2. QUARTZ: for short wavelength settings (UVR: below 340 nm)

Question 41

1. ROUND – ______ 2. SQUARE & RECTANGLE – __________________ PROPER HANDLING: No ___________, no _____________, and no ___________________

Answer 41

1. ROUND – glass 2. SQUARE & RECTANGLE – quartz/plastic PROPER HANDLING: No scratches, no detergents, and no sterilization

Question 42

For transfer of smaller volumes (<500ul) in blood gas analyis or in separationtechniques (chromatography, electrophoresis).

Answer 42

Syringes

Question 43

SPECIAL GLASSWARES:

Answer 43

1. Colored and Opal Glasses 2. Coated Glass 3. Optical Glass 4. Glass Ceramics 5. Radiation-Absorbing Glass

Question 44

 has metallic oxides; used for filters and light bulbs

Answer 44

Colored and Opal Glasses

Question 45

 has a thin, metallic oxide permanently fire-bonded to its surface; canconductelectricity  has electronic applications as a heat shield to protect against infraredlight andasan electrostatic shield to carry off charges

Answer 45

Coated Glass

Question 46

 made of soda lime, lead and borosilicate  has a high optical activity; prisms, lenses and optical mirrors

Answer 46

Optical Glass

Question 47

 with high thermal resistance, chemical stability and corrosion resistance  for hot plates, table tops and heat exchangers

Answer 47

Glass Ceramics

Question 48

made of soda lime and lead

Answer 48

Radiation-Absorbing Glass

Question 49

 Clear and rigid; not autoclavable  Used for disposable wares

Answer 49

Polystyrene (PS)

Question 50

 Not recommended for use with acids, aldehydes, ketones, ethers, hydrocarbonsor essential oils.  Alcohols and bases can be used but not to be stored longer than 24 hours.

Answer 50

Polystyrene (PS)

Question 51

 Chemically resistant to most substances except for aldehydes, amines, ethers, hydrocarbons and essential oils  ____________________________: translucent and flexible; not autoclavable

Answer 51

Polyethylene Conventional Polyethylene (CPE)

Question 52

 Has the same chemical resistant as polyethylene  Translucent and rigid; autoclavable; Used for screw-cap closures

Answer 52

Polypropylene (PP)

Question 53

 Resin that has excellent chemical resistance to almost all chemicals in the lab.  Clear, translucent and flexible; autoclavable  Used for stopcocks, wash bottles and tubings

Answer 53

Teflon

Question 54

Translucent and flexible; autoclavable; used for tubings

Answer 54

Tygon

Question 55

 Very susceptible to damage by most chemicals. Resistant to water, aqueous salts and inorganic acids for a long period.  Very clear and rigid; autoclavable  Used for __________, _________________

Answer 55

Polycarbonate (PC) carboys, test tube racks