INSTRUMENTATION

Question 1

When a beam of light passes through a solution, some of the light may be absorbed by the molecules of the substance in the solution, while the rest is transmitted?

Answer 1

Spectrophotometer

Question 2

It measures the quantity of light reflected by a liquid sample that has been dispensed onto a grainy or fibrous solid support?

Answer 2

Reflectometry

Question 3

What is the light emission from a singlet excited state called? (Immediate)

Answer 3

Fluorescence

Question 4

Compounds absorb electromagnetic radiation, become excited, and return to an energy level lower than or equal to their original level

Answer 4

Luminescence

Question 5

Light emission from an excited triplet state is called? (Gradual)

Answer 5

Phosphorescence [ when light is hurred off (ASTERGLOW) ]

Question 6

The emission of light is created from a chemical or electrochemical reaction

Answer 6

Chemiluminescence

Question 7

The emission of light is from the absorption of electromagnetic energy

Answer 7

Fluorescence & Phosphorescence

Question 8

Light scattering

Answer 8

Nephelometry

Question 9

Light refraction

Answer 9

Refractometry

Question 10

Light blocking/ Unscattered light (180°)

Answer 10

Turbidimetry

Question 11

Based on colligative properties. Measurement of the osmolality of an aqueous solution such as serum, plasma, or urine

Answer 11

Osmometry

Question 12

Outer fluid stream creates a laminar flow forcing the specimen to the center, and results in a single-file alignment of the individual cells

Answer 12

Hydrodynamic focusing

Question 13

Measures multiple properties of cells suspended in a moving fluid medium

Answer 13

Flor cytometry

Question 15

Proportional to cell size

Answer 15

Forward scattering

Question 16

Related to cell granularity and nuclear irregularity

Answer 16

Side Angle/ Right angle Scatter

Question 17

Green fluorescence

Answer 17

Means that the dye FLUORESCEIN was used as a marker

Question 18

Red fluorescence

Answer 18

Means that a dye such PHYCOERYTHRIN was used as the contrasting marker

Question 19

Red fluorescence

Answer 19

Means that a dye

Question 20

Involves measurement of the current or voltage generated by the activity of specific ions

Answer 20

Electrochemistry

Question 21

This measure of potential (voltage) between two electrodes in a solution forms the basis for a variety of procedures for measuring analyte concentration. Used in PCO2 and pH electrodes

Answer 21

Potentiometry (zero current)

Question 23

Measures the quantity of electricity (in coulombs) needed to convert an analyte to a different oxidation state

Answer 23

Coulometry

Question 24

The measurement of the current flow produced by an oxidation-reduction reaction
Used in PO2 electrodes (polarography)

Answer 24

Amperometry (current flow redox)

Question 25

A potential is applied to an electrochemical cell and the resulting current is measured

Answer 25

Voltammetry (net current)

Question 26

An electrochemical technique used to measure heavy metals such as lead

Answer 26

Anodic stripping voltammetry

Question 28

A measure of the ability of a solution to carry an electrical current

Answer 28

Conductance

Question 29

Based on the change in electrical resistance across an aperture when a particle in conductive liquid passes through this aperture

Answer 29

Electronic impedance

Question 31

The separation of charged compounds based on their electrical charge

Answer 31

Electrophoresis

Question 32

Absorbance of the stain on a support medium

Answer 32

Densitometry