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histupar lab > Instrumentation in Histopathology (MICROTOMY) > Flashcards

Instrumentation in Histopathology (MICROTOMY) Flashcards

(61 cards)

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1
Q

When there is rapid diagnosis

A

Freezing Microtome

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2
Q

temperature in a cryostat

A

5 to -30°C
(-20°C)

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3
Q

When histological demonstration of fat is needed

A

Freezing Microtome

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4
Q

Most dangerous type of microtome

A

SLIDING MICROTOME

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5
Q

Especially recommended for cutting extremely hard and
rough tissue blocks

A

SLIDING MICROTOME

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6
Q

Comparatively more stable than the sliding microtome
seen previously

A

Base-sledge Microtome

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7
Q

continuous sections

A

Serial sections

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8
Q

When sensitive tissue constituents to be studies are
damages are destroyed by heat

A

Freezing Microtome

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9
Q

Consists of microtome kept inside a cold chamber which
has been maintained at a temperature between -5 to -30°C (-20°C) by an adjustable thermostat

A

Cryostat

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10
Q

It has a movable exposed knife (moves up/down)

A

SLIDING MICROTOME

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11
Q

When certain neurological structures are to be studied

A

Freezing Microtome

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12
Q

Also known as sectioning

A

MICROTOMY

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13
Q

Dual purpose microtome can produce:
■ Rapid freezing sections for RFS
■ Routine paraffin sections for routine histopathology
lab tests

A

Computerized Microtome

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14
Q

Can cut sections of 4μ with ease

A

Cryostat

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15
Q

The blade is placed in a blade-up position which is relatively dangerous

A

ROTARY MICROTOME

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16
Q

Causes reciprocal motion of the knife over the block

A

flywheel

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17
Q

must be brushed away with soft brush and not allowed to stay in the microtome

A

accumulated paraffin and small pieces of tissues

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18
Q

Most commonly used for rapid preparation of urgent tissue biopsies for intraoperative diagnosis

A

cryostat

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19
Q

2 kinds of sliding microtome

A
  • Base-sledge Microtome
  • Standard sliding microtome
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20
Q

Consist of a heavy base and two arms

A

ROCKING MICROTOME

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21
Q

Cuts the block at a predetermined thickness. It
produces tissue ribbons that are connected to each
other

A

Knife

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22
Q

Invented by Queckett in 1848

A

FREEZING MICROTOME

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23
Q

After drying the machine and knifeh older, the parts should be wiped with

A

xylol

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24
Q

More dangerous than Base-sledge

A

Standard Sliding Microtome

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25
Simplest among the different types of microtome
ROCKING MICROTOME
26
cryostat Provides a means of preparing thin sections of fresh frozen tissues for ________________ or _____________
- fluorescent antibody staining techniques (immunohistochemistry) - histochemical enzyme studies (microstructure and enzyme/antigen integrity)
27
Most common type used for both routine and research laboratories
ROTARY Microtome
28
Line up the tissue block in the proper position
Adjustment screws
29
Difficulty of reorienting the block
ROCKING MICROTOME
30
Developed by Adams in 1789
SLIDING MICROTOME
31
Developed mainly for cutting celloidin-embedded tissue blocks
Standard Sliding Microtome
32
Designed for non-contact sectioning inside biological tissues without causing thermal damage
Laser Microtome
33
Can be used for paraffin wax and for tougher embedding materials like celloidin (resinous / plastic-like medium) and nitrocellulose (some kind of polymer)
SLIDING MICROTOME
34
Primarily used for cutting tissue sections at 0.5 micra for electron microscopy.
Ultrathin Microtome
35
are used to mount tissues in the Cryotome
OCT
36
The knife used for cutting the ultrathin sections consists mainly of selected fragments of broken plate glass.
37
Consist of two movable pillars holding the adjustable knife clamps, allowing the knife to be set at an angle for cutting celloidin sections
Base-sledge Microtome
38
cryostat is Capable of freezing fresh tissue within how many minutes
2-3 minutes
39
or lowering temperature of the knife to facilitate sectioning in frezzing microtome
second cooling device
40
in Ultrathin Microtome, The specimen used is small, fixed in ___________, and embedded in ______
- osmium tetroxide - plastic
41
Suited for sectioning specimens embedded in all forms of media
Base-sledge Microtome
42
The stage for block holder is hollow and perforated around its perimeter, attached to a reinforced flexible lead pipe through which carbon dioxide (freezing agent) passes from a cylinder
FREEZING MICROTOME
43
Designed to cut sections from very hard materials like undecalcified bone, teeth, glass, and ceramics
Saw Microtome
44
Restrictions in size of tissue block that can be cut
ROCKING MICROTOME
45
Invented by Minot in 1885-1886
Minot Microtome
46
is brought into contact with, and turns a ratchet feed wheel connected to a micrometer screw, which in turn rotated, moving the tissue block at a predetermined distance towards the knife for cutting sections at uniform thickness
spring balance teeth or pawl
47
Invented by Paldwell Trefall in 1881
Cambridge rocking microtome
48
The block remains stationary while the knife is moved backward and forward during the process of sectioning
Standard Sliding Microtome
49
Operated by the rotation of the flywheel
ROTARY MICROTOME
50
sometimes included
Copper mesh grid
51
in the ROTARY Microtome, the Thickness of the section being automatically regulated by the
ratchet feed wheel
52
Favored in laboratories where very hard tissue or large blocks are usually sectioned.
Base-sledge Microtome
53
Uses high-speed vibrations produced by safety razor blades which provide the cutting power, allowing the resultant cut to be made with less pressure than would the stationary blade be
Vibrating Microtome
54
Mostly used for rigid botanical materials
Hand Microtome
55
allows the release of rapid intermittent burst of carbon dioxide in freezing microtome
simple lever operated valve
56
Adjust the thickness for the sections
Adjustment screws
57
Basic instrument used that is capable of cutting section at a predetermined thickness by sliding the block into a cutting tool which is fixed and attached to the machine
MICROTOME
58
Not recommended for serial sections because tissues are cut in slightly curved planes
ROCKING MICROTOME
59
Holds the tissue
Chuck/block/tissue block
60
The knife and the block holder are brought together by upward and vertical motions
ROTARY MICROTOME
61
Used to cut undehydrated tissues in a frozen state
Freezing Microtome

histupar lab (18 decks)

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