Intro I (Reviewer) (RVSP) Flashcards
(20 cards)
Essential part of a microtome; for actual cutting of tissue sections
Knife carrier & knife
Essential part of a microtome; where the tissue is held in position
Block holder
Essential part of a microtome; used to line up the tissue block in proper position with the knife; used to adjust the thickness of the tissue for successive sections
Ratchet feed wheel
Pawl
Adjustment screws
Type of microtome; simplest microtome
Rocking Microtome
Inventor of the rocking microtome
Caldwell Trefall
Inventor of the Rotary Microtome
Minot
Inventor of the Freezing Microtome
Queckett
Type of microtome; capable of cutting sections for electron microscopy
Ultra-thin Microtome
Thickness of sections cut by ultra-thin microtomes
0.5 μ
Type of microtome recommended for cutting extremely hard blocks
Sliding/Base Sledge Microtome
Inventor of the Sliding/Base Sledge Microtome
Adams
The histology technician’s tool of his profession
Microtome Knife
Types of microtomes that use biconcave microtome knives
Rocking microtome
Rotary microtome
Carbon dioxide-freezing microtome
The plane-concave microtome knife with lesser concavity is used for
Celloidin sections
The plane-concave microtome knife with more concavity is used for
Paraffin sections on the sledge, rotary or rocking microtome
The plane-wedge microtome knife is routinely used for
Frozen sections and extremely hard specimens embedded in paraffin wax; also for paraffin blocks cut on the rotary microtome
3 types of microtome knives
Biconcave
Plane-concave
Plane-wedge
2 chemicals that can be used to clean microtome knives
Xylene
Toluene
Temperature of Cryostat
-20°C
Machine on which sections are cut
Microtome
