Intro to Bacterial UTI's

Question 1

5 major defenses of the urinary tract?

Answer 1

1. Flushing of urine
2. IgA mucosa
3. Urine chemistry
4. Epithelial surface proteins
5. Normal flora

Question 2

What are the 3 components of urine chemistry?

Answer 2

1. acidity
2. lysozymes- lyse peptidoglycan
3. lactoferrin- take iron away from bacteria

Question 3

3 major threats to the urinary system?

Answer 3

1. normal biota of the body- usually from GI tract
2. Catheter insertion
3. glomerulonephritis caused by strep pyogenes (skin or strep throat)

Question 4

Top 5 causative agents of UTIs

Answer 4

1. Escherichia coli
2. Staph saprophyticus
3. Klebsiella
4. Proteus mirabilis
5. Enterococcus

Question 5

Selective media

Answer 5

contains compounds that selectively inhibit growth of some microbes but not others

Question 6

Differential Media

Answer 6

contains an indicator usually a dye that detects particular chemical reactions occurring during growth

Question 7

Culture media

Answer 7

supply the nutritional needs of microorganisms and can be either chemically defined or undefined; defined medium or complex medium

Question 8

Catalase test

Answer 8

Aerobic and facultative anaerobic organisms produce reactive oxygen species during normal metabolism= H2O2 and O2-;

Question 9

Novobiocin Ab

Answer 9

Inhibits DNA gyrase in susceptible microorganism; sensitive you would see a clearing (Staph epi) or resistant which you won’t see any inhibition (Staph Saphrophyticus)

Question 10

Staph saprophyticus is…

Answer 10

Catalase +, coagulase -, Novobiocin resistant

Question 11

Staph aureus is catalase + or -

Answer 11

+

Question 12

gamma hemolysis looks like what on the culture media?

Answer 12

none

Question 13

B-hemolysis looks like what on the culture media?

Answer 13

gold colonies (staph aureus)