Intro to bacteriology

Question 1

smallest bacteria

Answer 1

Mycoplasma spp.

Question 2

largest bacteria

Answer 2

Thiomargarita namibiensis

Question 3

largest medically important bacteria

Answer 3

Borrelia Burgdoferi (lyme disease)

Question 4

bacteria with no cell wall

Answer 4

Mycoplasma spp. and Ureaplasma spp.

Question 5

steps of gram staining

Answer 5

VIAS

• crystal violet
• iodine
• acetone/alcohol (95%)
• safranin

Question 6

all cocci are positive except:

Answer 6

Branhemella
Neisseria
Veilonella

Question 7

all bacilli are negative except:

Answer 7

Bacillus, Clostridium (spore forming)
Actimomyces, Nocardia (fungi like)
MYCObacterium, CORYNEbactrium, PROPIONObacterium
Erysipelothrix, Listeria

Question 8

steps in acid fast staining

Answer 8

Carbol-fuchsin
acid alcohol
methylene blue/malachite green

Question 9

mordant of Ziehl-nelseen

Answer 9

steam

Question 10

mordant of kinyoun

Answer 10

tergitol

Question 11

method used for Leprosy

Answer 11

FITE-FARACO method

Question 12

important enzyme in obligate aerobe

Answer 12

superoxide dismutase
peroxidase
catalase

Question 13

example of obligate anaerobe

Answer 13

Actinomyces
Bacteriodes
Clostridium

Question 14

example of capnohilic bacteria

Answer 14

(HACEK)
Haemophilus
Acinobacter
Cardiobacterium
Eikenella
Kingella

Question 15

Which has higher toxicity exotoxin or endotoxin?

Answer 15

Exotoxin - high toxicity

Question 16

Vaccine from exotoxin

Answer 16

Toxoids

Question 17

An exotoxin gram positive bacteria that is NOT rapidly destroyed at 60C?

Answer 17

Staphylococcus enterotoxin

Question 18

What are the disease caused by: enterotoxin & endotoxin?

Answer 18

Exo: tetanus, botulism
Endo: Meningococcemia

Question 19

Test that detects endotoxin the reagent used

Answer 19

Limulus lysate test

Reag: blood of horse-shoe crab (limulus polyphemus)

Question 20

Principle of limulus lysate test and its positive indication?

Answer 20

Amebocyte ( WBC) + endotoxin = clumping (blue color)

Question 21

Indicator of DNASE test?

Positive result?

Answer 21

Methyl green

Clearing (Staph. aureus, Moraxella, Serratia)

Question 22

Mannitol composition?
Carbohydrates:
Indicator:
Nacl content:

Answer 22

D-mannitol
Phenol red
7.5-10% NaCl (staphylococcus aureus)

Question 23

Group B streptococcus

Answer 23

Todd Hewitt broth
LIM broth
Granada agar

Question 24

Group D strep

Answer 24

Bile Esculin Agar (BEA)

Question 25

Clostridium perfringes

Answer 25

Egg yolk agar

Question 26

Clostridium difficille

Answer 26

Cycloserine-cefoxitin agar

Question 27

Tellurite Tinsdale Pai coagulate egg LSS medium These are specific for what bacteria

Answer 27

Corynebacterium diptheria

Question 28

Listeria monocytogenes

Answer 28

Mcbridge agar

Question 29

Tomato agar is commonly used for what bacteria?

Answer 29

Lactobacillus vaginalis

Question 30

Differential agar for Neisseria spp.

Answer 30

Cystine trypticase agar

Question 31

Bordatella pertussis

Answer 31

Bordet gengou | Regan lowe

Question 32

Agar for Legionella

Answer 32

Charchoal yeast agar

Question 33

Haemophilus spp.

Answer 33

Potato yeast agar

Question 34

E.coli

Answer 34

EMB (eosin methylene blue agar

Question 35

Salmonella and shigella

Answer 35

Xylose lysine dextrose agar (XLD)

Question 36

Thiosulfate citrate bile salt agar Acid peptone agar Used for what bacteria?

Answer 36

Vbrio spp.

Question 37

Campylobacter jejuni

Answer 37

skirrow’s agar

Question 38

Cetrimide agar is a culture media for

Answer 38

Pseudomonas aeruginosa

Question 39

Castadena bottle for what species of bacteria?

Answer 39

Brucella spp.

Question 40

What bacteria should be inoculated in a cysteine-glucose-blood agar

Answer 40

Franciscella spp.

Question 41

Eaton’s medium is a culture media choice for what bacteria

Answer 41

Mycoplasma

Question 42

Culture media for genital mycoplasma

Answer 42

Shepard agar

Question 43

Barbour stoenner kelly is an isolation media for

Answer 43

Borrelia burgdorferi

Question 44

What are the different culture media to use for leptospira spp.

Answer 44

EMJH (Ellinghausen-McCullough- Johnson-Harris) Fletcher medium Stuart medium

Question 45

Cycloheximide agar and Mccoy agar are culture media for:

Answer 45

Chlamydia

Question 46

Gardnerella

Answer 46

Human blood bilayer tween medium

Question 47

Yersinia enterocolitica

Answer 47

CIN (Cefsulodin-irgasan-novobiocin)

Question 48

Selective media for B. Anthracis

Answer 48

Polymixin Lysozyme EDTA thallous acetate (PLET)

Question 49

What virulence factor causes flaccid paralysis?

Answer 49

Botulinum neurotoxin

Question 50

Erythromycin and tetracycline ointment is associated to what disease?

Answer 50

Opthalmia neonaturum

Question 51

What type of hemolysis is present in C. Perfringes?

Answer 51

``` Double hemolysis in Bap Theta toxin (inner) Alpha toxin (outer) ```

Question 52

What disorder is associated with infant botulism which caused by bees and honey

Answer 52

Floppy baby syndrome

Question 53

What are the manifestation for tetanus disease

Answer 53

Trismus (locked jaw) Risus sardonicus (evil grin) Opisthotonus (tensed back)