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MICROBIOLOGY > Intro to bacteriology > Flashcards

Intro to bacteriology Flashcards

1
Q

smallest bacteria

A

Mycoplasma spp.

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2
Q

largest bacteria

A

Thiomargarita namibiensis

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3
Q

largest medically important bacteria

A

Borrelia Burgdoferi (lyme disease)

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4
Q

bacteria with no cell wall

A

Mycoplasma spp. and Ureaplasma spp.

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5
Q

steps of gram staining

A

VIAS

  • crystal violet
  • iodine
  • acetone/alcohol (95%)
  • safranin
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6
Q

all cocci are positive except:

A

Branhemella
Neisseria
Veilonella

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7
Q

all bacilli are negative except:

A

Bacillus, Clostridium (spore forming)
Actimomyces, Nocardia (fungi like)
MYCObacterium, CORYNEbactrium, PROPIONObacterium
Erysipelothrix, Listeria

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8
Q

steps in acid fast staining

A

Carbol-fuchsin
acid alcohol
methylene blue/malachite green

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9
Q

mordant of Ziehl-nelseen

A

steam

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10
Q

mordant of kinyoun

A

tergitol

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11
Q

method used for Leprosy

A

FITE-FARACO method

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12
Q

important enzyme in obligate aerobe

A

superoxide dismutase
peroxidase
catalase

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13
Q

example of obligate anaerobe

A

Actinomyces
Bacteriodes
Clostridium

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14
Q

example of capnohilic bacteria

A 
(HACEK)
Haemophilus
Acinobacter
Cardiobacterium
Eikenella
Kingella
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15
Q

Which has higher toxicity exotoxin or endotoxin?

A

Exotoxin - high toxicity

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16
Q

Vaccine from exotoxin

A

Toxoids

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17
Q

An exotoxin gram positive bacteria that is NOT rapidly destroyed at 60C?

A

Staphylococcus enterotoxin

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18
Q

What are the disease caused by: enterotoxin & endotoxin?

A

Exo: tetanus, botulism
Endo: Meningococcemia

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19
Q

Test that detects endotoxin the reagent used

A

Limulus lysate test

Reag: blood of horse-shoe crab (limulus polyphemus)

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20
Q

Principle of limulus lysate test and its positive indication?

A

Amebocyte ( WBC) + endotoxin = clumping (blue color)

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21
Q

Indicator of DNASE test?

Positive result?

A

Methyl green

Clearing (Staph. aureus, Moraxella, Serratia)

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22
Q

Mannitol composition?
Carbohydrates:
Indicator:
Nacl content:

A

D-mannitol
Phenol red
7.5-10% NaCl (staphylococcus aureus)

23
Q

Group B streptococcus

A

Todd Hewitt broth
LIM broth
Granada agar

24
Q

Group D strep

A

Bile Esculin Agar (BEA)

25
Q

Clostridium perfringes

A

Egg yolk agar

26
Q

Clostridium difficille

A

Cycloserine-cefoxitin agar

27
Q

Tellurite
Tinsdale
Pai coagulate egg
LSS medium

These are specific for what bacteria

A

Corynebacterium diptheria

28
Q

Listeria monocytogenes

A

Mcbridge agar

29
Q

Tomato agar is commonly used for what bacteria?

A

Lactobacillus vaginalis

30
Q

Differential agar for Neisseria spp.

A

Cystine trypticase agar

31
Q

Bordatella pertussis

A

Bordet gengou

Regan lowe

32
Q

Agar for Legionella

A

Charchoal yeast agar

33
Q

Haemophilus spp.

A

Potato yeast agar

34
Q

E.coli

A

EMB (eosin methylene blue agar

35
Q

Salmonella and shigella

A

Xylose lysine dextrose agar (XLD)

36
Q

Thiosulfate citrate bile salt agar
Acid peptone agar

Used for what bacteria?

A

Vbrio spp.

37
Q

Campylobacter jejuni

A

skirrow’s agar

38
Q

Cetrimide agar is a culture media for

A

Pseudomonas aeruginosa

39
Q

Castadena bottle for what species of bacteria?

A

Brucella spp.

40
Q

What bacteria should be inoculated in a cysteine-glucose-blood agar

A

Franciscella spp.

41
Q

Eaton’s medium is a culture media choice for what bacteria

A

Mycoplasma

42
Q

Culture media for genital mycoplasma

A

Shepard agar

43
Q

Barbour stoenner kelly is an isolation media for

A

Borrelia burgdorferi

44
Q

What are the different culture media to use for leptospira spp.

A

EMJH (Ellinghausen-McCullough- Johnson-Harris)
Fletcher medium
Stuart medium

45
Q

Cycloheximide agar and Mccoy agar are culture media for:

A

Chlamydia

46
Q

Gardnerella

A

Human blood bilayer tween medium

47
Q

Yersinia enterocolitica

A

CIN (Cefsulodin-irgasan-novobiocin)

48
Q

Selective media for B. Anthracis

A

Polymixin Lysozyme EDTA thallous acetate (PLET)

49
Q

What virulence factor causes flaccid paralysis?

A

Botulinum neurotoxin

50
Q

Erythromycin and tetracycline ointment is associated to what disease?

A

Opthalmia neonaturum

51
Q

What type of hemolysis is present in C. Perfringes?

A 
Double hemolysis in Bap
Theta toxin (inner) 
Alpha toxin (outer)
52
Q

What disorder is associated with infant botulism which caused by bees and honey

A

Floppy baby syndrome

53
Q

What are the manifestation for tetanus disease

A

Trismus (locked jaw)
Risus sardonicus (evil grin)
Opisthotonus (tensed back)

MICROBIOLOGY (5 decks)

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