Intro to SEM - Sample Preparation Flashcards
What are 6 types of Electron Microscopes?
What does an SEM do? and how does it work?
What is a TEM? and how does it work?
What is the difference between SEM and TEM?
What is the Resolotion of the following:
- Human Eye
- Light Scope
- SEM
- TEM
What is the difference in sample preperation between a biological sample and non-biological sample?
What is important in sample preparation for an SEM?
Why do we clean the surface of the specimen? and what techniques are used?
Why do we fixate a specimen? Why is fixation so important?
What is a common fixative?
Why do we dehydrate biological samples?
How do we dehydrate a sample?
Describe the following drying methoda:
- Air Drying
- Critical Point Drying (CPD)
- Freeze Drying
What is the Critical Point? and describe the CPD process?
Critical Point = temperature/pressure combination specific for the transitional fluid at which point a transition occurs and the density of the liquid phase equals that of the vapor phase
How do we freeze dry a speciment?
What is important to consider when choosing a stub for mounting a specimen?
What is important to consider when selecting an adhesive for mounting specimen to a sample?
What is important about orientating the specimen correctly on the stub?
Why do we coat our samples once mounted on the stub?
What does the coating material do? and what are the different coating techniques?
Describe Sputter Coating:
What factors do we consider when sputter coating?
