introduction into host cell Flashcards Preview

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Flashcards in introduction into host cell Deck (5)
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1
Q

why are various methods required to aid the process of DNA entering host cell?

A

DNA does not easily cross the recipient’s cell surface membrane

2
Q

Ca2+ and heat shock

A
  • temp of culture lowered to 0 degrees Celsius then increased quickly to 40 degrees Celsius
    helping to increase rate at which plasmids are taken up by bacterial cells
3
Q

electroporation

A

high voltage pulse disrupts plasma membrane

4
Q

electrofusion

A

electric fields help to introduce plasmids

5
Q

transfection

A

bacteria can be packaged into bacteriophage which can transfect host cell