ISBB

Question 1

Normal serum proteins that can rapidly increase during an inflammation

Answer 1

Acute Phase Reactants

Question 2

Concentration of CRP to be positive

Answer 2

0.6 mg/dL

Question 3

Can be elevated during surgery and pregnancy

Answer 3

CRP

Question 4

How much sample is needed in CRP/ASO/RF test

Answer 4

50 ul

Question 5

Most widely used serological test for the detection of Group A hemolytic streptococcus infection

Answer 5

Anti-Streptolysin O Test

Question 6

After how many minutes should CRP/ASO/RF be observed during testing?

Answer 6

2 minutes

Question 7

Most common autoimmune disease; involves in the production of IgM or IgG antibodies against IgG

Answer 7

Rheumatoid Arthritis

Question 8

Rheumatoid factors are directed against the ________ of the IgG molecules

Answer 8

Fc fragment

Question 9

HIV strain most common in the USA

Answer 9

HIV-1

Question 10

Test kit detects gp 36 for this type of HIV

Answer 10

HIV-2

Question 11

Principle of HIV test kits

Answer 11

Lateral Flow Immunochromatography

Question 12

Foundation of ISBB

Answer 12

Antigen-Antibody Interactions

Question 13

Antigenicity Factors

Answer 13

Foreignness, Molecular Weight, Complexity

Question 14

Most immunogenic composition

Answer 14

Proteins

Question 15

Antibody class that exists as dimers in secretions

Answer 15

IgA

Question 16

Antibody class conducive for agglutination

Answer 16

IgM

Question 17

Prepared to provide accurate serum:cell ratio

Answer 17

Red Cell Suspensions

Question 18

Excess antibody

Answer 18

Prozone

Question 19

Formula for pRBC volume

Answer 19

%RBC * Total Volume

Question 20

Grade agglutination: 2+

Answer 20

medium agglutinates

Question 21

Simplest and most common application of Ag-Ab interaction

Answer 21

ABO/Rh Typing

Question 22

What is the recessive gene among ABO genes?

Answer 22

O gene

Question 23

Precursor antigen

Answer 23

H antigen

Question 24

RBC structure without antigen

Answer 24

glucose-galactose- N-acetylglucosamine -galactose

Question 25

Immunodominant sugar for A antigen

Answer 25

N-acetylgalactosamine

Question 26

If A antigen in present in the RBC, what antibody is present in the serum?

Answer 26

Anti-B

Question 27

Sample: red cells/ whole blood Reagent: antisera Tests for: antigens on RBCs

Answer 27

Forward Typing

Question 28

Anti-A - 4+ Anti-B - 4+ A Cells - 0 B Cells - 0

Answer 28

AB

Question 29

Problem with antibodies in reverse typing

Answer 29

Group I Discrepancies

Question 30

Less common discrepancy

Answer 30

Group II

Question 31

Seen in: Multiple myeloma, Waldenstrom's macroglobulinemia, Wharton's jelly

Answer 31

Group III

Question 32

Agglutinates A1 or A1B cells, but not A2

Answer 32

Dolichos biflorus

Question 33

``` Expected Panel Result: A Cells - 4+ B Cells - 4+ O cells - 4+ Anti-A - 0 Anti-B - 0 Autocontrol - 0 ```

Answer 33

Bombay phenotype

Question 34

often associated with the diseases of the digestive tract

Answer 34

Acquired B phenomenon

Question 35

Unique Discrepancies

Answer 35

1. Presence of A/B subgroups 2. Bombay Phenotypes 3. Acquired B Phenomenon

Question 36

ABO Discrepancies: | Mixed Field

Answer 36

Group O transfusions, stem cell transplant

Question 37

7 Physical Examination of Donors

Answer 37

Age, Weight, Pulse, Blood Pressure, Temperature, Hemoglobin, Hematocrit

Question 38

What does the primary capture antibody in test kits interact with?

Answer 38

Analyte (Ag)

Question 39

T or F: Presence of two bands in HIV test kits indicates co-infection

Answer 39

False

Question 40

T or F: In the absence of HbsAg, presence of Anti-Hbc indicates immunity.

Answer 40

False. It indicates recovery.

Question 41

Marker of immunity

Answer 41

Anti-Hbs

Question 42

RPR result: | large and medium black floccules against white background

Answer 42

Reactive

Question 43

Often used to obtain titers

Answer 43

Serial dilution

Question 44

Blood bag anticoagulant component that is a substitute for ATP production

Answer 44

Dextrose

Question 45

Some are termed as non-agglutination / incomplete antibodies

Answer 45

IgG

Question 46

Last check of ABO compatibility

Answer 46

Crossmatching

Question 47

Phase that detects ABO incompatibilities

Answer 47

Immediate Saline Spin Phase

Question 48

Causes of Positive Crossmatch

Answer 48

1. Incorrect ABO Grouping 2. Presence of alloantibodies (ac - neg) 3. Presence of autoantibodies (ac - pos) 4. Proteins coating donor RBCs 5. Abnormalities in plasma

Question 49

How many minutes should RPR be read? What should be observed?

Answer 49

Observed for flocculation within 8 minutes.

Question 50

Antigen detected within first 1-9 days of dengue infection

Answer 50

NS1 antigen