Isolation and Cultivation

Question 1

Indicative of purity

Answer 1

No. of colonies (>1 = contaminated)

Question 2

What comprise the dry weight of a cell?

Answer 2

Carbon, nitrogen, phosphorous, vitamins, trace elements

Question 3

Imprecise nutrition composition

Answer 3

Complex Culture Media

Question 4

Processes in nitrogen sources

Answer 4

Protein synthesis & amino acid production

Question 5

Has additional nutrients (eg. blood, serum)

Answer 5

Enriched Medium

Question 6

CO2 is reduced to glucose

Answer 6

Autotrophic

Question 7

What is calcium for

Answer 7

Cell wall stability, heat stabilization for endospores

Question 8

Common elements

Answer 8

P, S, K, Mg

Question 9

EMB inhibits growth of

Answer 9

Gram +

Question 10

Contains compounds that inhibit the growth of one bacterial group while supporting the growth of another

Answer 10

Selective Medium

Question 11

Cofactors in enzymatic reactions

Answer 11

Trace & Vitamins

Question 12

Transparent outside

Answer 12

Capability to form biofilm

Question 13

Color of EMB

Answer 13

Green or brown

Question 14

Use fir liquid media

Answer 14

Preservation

Question 15

Color of E. Coli

Answer 15

Metallic green

Question 16

Characteristics that can help in determining unknown organism

Answer 16

Form, margin, elevation, texture, size, color

Question 17

Whole RBC

Answer 17

Blood Plate Agar

Question 18

Chocolate agar is used for isolating

Answer 18

Neisseria and H. influenzae

Question 19

RBC that have broken up

Answer 19

Chocolate Agar

Question 20

Necessary for synthesis of new cell materials

Answer 20

Carbon sources

Question 21

Detect biochemical reactions that may occur (by dyes changing color)

Answer 21

Differential Culture Medium

Question 22

Agar is composed of how much liquid vol.

Answer 22

1.5-3%

Question 23

Increased enzyme synthesis (uptake of limited nutrients, replacement of limited in place of available)

Answer 23

Adaptation

Question 24

Prefers glucose, lactose

Answer 24

Heterotrophic

Question 25

Also detects lactose fermentation

Answer 25

EMB

Question 26

Blood plate agar is used to isolate

Answer 26

S. haemolyticus (which is infectious and has different variants)

Question 27

Most preferred by certain bacteria

Answer 27

Glucose

Question 28

MSA is composed of (blank) and detects (blank)

Answer 28

Phenol red, mannitol fermentation

Question 29

Use for solid media

Answer 29

Isolation & purification

Question 30

MacConkey (MAC) agar is composed of (blank) and inhibits (blank)

Answer 30

Bile salts, Gram +

Question 31

Precise amounts of highly purified organic/inorganic components

Answer 31

Defined Culture Media

Question 32

Complex culture media is usually in the color

Answer 32

Yellow

Question 33

Ccomposition of MAC and what does it detect

Answer 33

Neutral red, lactose fermentation

Question 34

Decrease in metabolic activity

Answer 34

Adaptation

Question 35

13% of the cell

Answer 35

Nitrogen sources: proteins, nucleic acid, etc.

Question 36

Composed of 7.5% NaCl and inhibits Gram - & salt sensitive Gram +

Answer 36

Mannitol Salt Agar (MSA)

Question 37

Where to get nitrogen

Answer 37

Tryptone, peptone

Question 38

True or false. Sodium content depends in the nutrient requirement of the bacteria.

Answer 38

True.