Isolation and Cultivation of Microorganisms Flashcards
1
Q
List
Essential elements as a percent of cell dry weight
7
A
- Carbon
- Oxygen
- Nitrogen
- Hydrogen
- Phosphorus
- Sulfur
- Selenium
2
Q
Composition of a Cell
4 | Bonus: Percentages
A
Water: 70-80% wet weight
Proteins: 55% dry weight
RNA: 20.5% dry
DNA: 3.1% dry
3
Q
Main kinds of sources
2
A
Carbon
Nitrogen
4
Q
Kinds of microorganisms (carbon sources)
2
A
Hetotrophic
Autotrophic
5
Q
- Microorganisms that require organic compounds as their source
- Ex. Glucose, lactose
A
Heterotrophic microorganisms
6
Q
- Microorganisms that use CO2 as carbon source
- no need to provide carbon source in medium
- CO2 is reduced to glucose
A
Autotrophic microorganisms
7
Q
Differentiate
Carbon Sources vs Nitrogen Sources
A
Carbon
* Necessary for synthesis of new cell materials
Nitrogen
* Necessary for protein synthesis and amino acid production
8
Q
What do trace elements and vitamins do?
A
- Act as cofactors in enzymatic reactions
- Some pathogens obtain vitamins from host
- Ex: Cu, Co, Zn, Mg, folic acid, vitamin B12, vitamin K
9
Q
List
Types of Culture Media
2
A
- Defined
- Complex
10
Q
- Media that contains precise amounts of highly purified organic/inorganic components
- Usu. colorless
A
Defined Media
11
Q
- Media that contains dehydrated digests of animal, plant, or microbial products
- Usu. yellow colored
- Contains imprecise nutritional composition
- Eg casein, yeast, beef soybeans
A
Complex
12
Q
Why is there glucose in culture media?
A
- organisms can utilize glucose as a carbon source
- whether it is defined/complex
13
Q
Kinds of Complex Media
5
A
- Enriched medium
- Selective Medium
- Differential medium
14
Q
Complex base w/ addtl nutrients (e.g. blood, serum, etc.)
A
Enriched medium
15
Q
List
Examples of Enriched Medium
2
A
- Chocolate agar
- Blood plate agar
