Flashcards in Jackson: Bacterial Structure and Metabolism Deck (36)
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Fermentation:
Incomplete glucose oxidation (anaerobic; produces acid or alcohol)
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TCA Cycle:
Acetyl CoA + citrate → NADH + FADH2
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ETC:
ETC: redox carriers in cytoplasmic membrane yield ATP
o Substrates → Dehydrogenases → Quinones → Cytochromes → Terminal oxidases
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Aerobic vs anaerobic vs Facultative
Examples
o Aerobic: terminal electron acceptor is oxygen (most efficient); Mycobacteria
o Anaerobic: use fermentation pathway; terminal acceptor is nitrate, nitrite, fumarate or sulfate; Clostridia
o Facultative: can use both of the above pathways; E.coli
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Lab Tests Based on Metabolic Reactions (4):
Catabolism of sugars:
H2S production detected as:
Oxidase test detects:
Catalase test checks for:
o Catabolism of sugars to produce acid
o H2S production detected as gas or FeS
o Oxidase test detects cytochrome oxidase c
o Catalase test checks for enzyme that breaks down toxic O2 during respiration
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