Ka 2 Dna Replication

Question 1

What’s the role of DNA polymerase in DNA replication?

Answer 1

Adds DNA nucleotides using complimentary base paring to the deoxyribose 3’ end of the new DNA stand which is forming.

Question 2

What does DNA polymerase need to start replication?

Answer 2

A primer

Question 3

What end can DNA polymerase add nucleotides to?

Answer 3

Deoxyribose (3’) end

Question 4

The role of DNA ligase

Answer 4

It joins the replicated fragment of DNA togethe

Question 5

Describe the process of DNA replication

Answer 5

The DNA unwinds and the hydrogen bonds are broken. Which gives u 2 strands. A primer is needed to start replication.
Leading strand: primer binds to the target strand, then DNA polymerase adds nucleotides to the 3’ end continuously and then forms of a sugar, phosphate backbone between nucleotides. Repeated until entire strand is replicated.
Lagging stand: the strand is replicated is fragments. Joined by Ligase. As DNA separates another fragment will connect until entire molecule is replicated.

Question 6

Stages in the pcr process

Answer 6

•Heat up 92-98c to separate strands
• cooled 50-65c to let primers to bind with target sequence
• heated between 70-80c for heat tolerant DNA polymerase to replicate the region of DNA
• which amplifies the target region of DNA

Question 7

Role of primer in the PCR process

Answer 7

The starting point for DNA replication!
they bind to a specific segment of DNA and signal the DNA polymerase to begin copying the DNA

Question 8

Explain the role of heat tolerant polymerase in a PCR process

Answer 8

It can withstand hot temp which helps the pcr process helps amplify DNA

Question 9

Uses of PCR

Answer 9

•crimes
• paternity uses
• diagnosis of disorders